Electroporation-induced siRNA precipitation obscures the efficiency of siRNA loading into extracellular vesicles.

By S. Kooijmans, S. Stremersch, K. Braeckmans, S. D. De Smedt, A. Hendrix, M. Wood, Raymond Schiffelers, K. Raemdonck, P. Vader. Journal of Controlled Release, 2013

606 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/7ac954808bcd9abcf1182347b1f7c8efd7fa1249

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cancer Research

1. Yan Luo
2. Ben Wang
3. Chuanjiang He
4. Shu Zheng
5. P. Vader
6. Jan Lötvall
7. Edit I. Buzás
8. S. Bhattacharyya

In Molecular Biology

1. C. Théry
2. P. Vader
3. Yan Luo
4. Ben Wang
5. Chuanjiang He
6. Shu Zheng
7. Jan Lötvall
8. Edit I. Buzás

Citations in the corpus

• Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper (2017, background): “Although RNA extraction kits usually give very low DNA contamination,[61] many EV-RNA researchers indicated that they regularly experience DNA contamination in EV-RNA preparations.”
• Exosome Theranostics: Biology and Translational Medicine (2018, methodology): “Electroporation and chemical treatment are the primary methods used to load exosomes with cargo, although the corresponding loading efficiencies are not desirable [179] (Figure 4).”
• Applying extracellular vesicles based therapeutics in clinical trials – an ISEV position paper (2015, background): “However, the efficacy of this exogenous method has not been fully demonstrated, and the initially reported loading efficiencies may have been overestimated due to the possible aggregation of siRNAs in the electroporation buffer (229).”