Plat-E: an efficient and stable system for transient packaging of retroviruses

By S. Morita, T. Kojima, T. Kitamura. Gene Therapy, 2000

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Scaffolding Adapter Grb2-Associated Binder 2 Requires Syk to Transmit Signals from FcεRI1 (2006, methodology): “PMXs-puro and MSCV-IRES-GFP retroviral plasmids were transfected into the ecotrophic packaging cell line PlateE (31) (provided by T. Kitamura, Tokyo University, Tokyo, Japan) using Fugene reagent (Roche).”
Germinal Centers (2017, methodology): “The preparation of 4-hydroxy-3-nitrophenylacetyl (NP)-CGG has been described before [5, 9].”
Trans-endocytosis of CD80 and CD86: a molecular basis for the cell extrinsic function of CTLA-4 (2011, methodology): “Retroviral superntants were prepared by transfecting retroviral vector into the packaging cell line Plat-E (32) using Lipofectamine 2000 (Invitrogen).”
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. (2006, methodology): “We introduced each of the 24 candidate genes into mouse embryonic ﬁbroblasts (MEFs) from Fbx15 b geo/ b geo embryos by retroviral transduction (Morita et al., 2000).”
Induction of pluripotent stem cells from adult human fibroblasts by defined factors. (2007, methodology): “As a control, we introduced GFP to mouse embryonic fibroblasts (MEF) with ecotropic retrovirus produced in PLAT-E packaging cells(Morita et al., 2000).”