One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.

By K. Datsenko, B. Wanner. Proceedings of the National Academy of Sciences of the United States of America, 2000

15,585 citations recorded by the source corpus.

Subfields: Ecology, Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/773cded6afb7b47c29d3eae21f7de4ae55babb33

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Citations in the corpus

Characterization of Six Lipoproteins in the σE Regulon (2005, methodology): “The chromosomal disruptions of the yeaY, yfgL, nlpB, yfeY, and yraP open reading frames were generated according to the procedure described in the work of Datsenko and Wanner (12) as follows: PCR products were generated by using several pairs of 56- to 70-nucleotide (nt)-long primers that included 3”
A highly sensitive selection method for directed evolution of homing endonucleases (2005, result): “This library was transformed into BW25141 (lacI q rrnBT14 DlacZWJ16 DphoBR580 hsdR514 DaraBADAH33 DrhaBADLD78 galU95 endABT333 uidA(DMluI)::pir recA1) (14,15) (a gift from W.”
Environmentally controlled invasion of cancer cells by engineered bacteria. (2006, methodology): “The cassette was amplified with oligonucleotides ca598F and ca600R and recombined into the genome of MC1061 by the methods described by Datsenko and Wanner.51 The kanamycin resistance cassette was eliminated by Flp-mediated recombination51 to generate an antibiotic-sensitive fim deletion strain.”
A System for the Continuous Directed Evolution of Biomolecules (2011, methodology): “coli S109 cells derived from DH10B by replacement of the proBA locus with the pir116 allele, as previously described.(31) To our knowledge, this modification was not required for PACE experiments with T7 RNAP.”
A common mechanism of cellular death induced by bactericidal antibiotics. (2007, neutral): “Removal of the kanamycin-resistance cassette was accomplished using the pcp20 plasmid (Datsenko and Wanner, 2000) (Table S4) and confirmed by PCR prior to experimentation.”
Integrating high-throughput and computational data elucidates bacterial networks (2004, methodology): “27 ), and all deletion strains were generated as describe”
Specialized Persister Cells and the Mechanism of Multidrug Tolerance in Escherichia coli (2004, methodology): “Knockout strains (KL310, KL311, and KL312), were constructed by the protocol described by Datsenko and Wanner (17). pKD13 was used as a PCR template with the primers described online at http://www.atsweb .neu.edu/lewislab/mechpersisters.html.”