Quantification on the LightCycler

By R. Rasmussen. 2001

936 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/76f0670faa53b59b7ae042c7494d5fe2dbf719be

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Citations in the corpus

Statistical analysis of real-time PCR data (2006, neutral): “Ramussen 2001 and Pfaffl 2001 [7,21].”
An improvement of the 2ˆ(-delta delta CT) method for quantitative real-time polymerase chain reaction data analysis. (2013, methodology): “Another method is the dilution method (23), which calculated the amplification rate E based on the slope of a linear curve of a series of dilutions (E =10-1/slope).”
Absolute and relative QPCR quantification of plasmid copy number in Escherichia coli. (2006, methodology): “From the slope of each standard curve, PCR amplification efficiency (E) was calculated according to the equation (Rasmussen, 2001): E = 10 −1/slope − 1 (2)”
Bacillus subtilis SQR 9 can control Fusarium wilt in cucumber by colonizing plant roots (2011, methodology): “Standard curves were generated to calculate PCR efficiency (E=10−1/slope−1, Rasmussen 2001) by plotting the threshold cycle (Ct value) for the standard DNA against the copy number as described by Jørgensen and Leser (2007).”
Growth improvement by probiotic in European sea bass juveniles (Dicentrarchus labrax, L.), with particular attention to IGF-1, myostatin and cortisol gene expression (2006, methodology): “The corresponding real-time PCR efficiencies were calculated according to the equation: E=10[−1/slope] (Rasmussen, 2001).”
Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR (2002, methodology): “…adequate methodologies like Threshold Cycle (18,19) where CP will be measured at constant fluorescence level and the Second Derivative Maximum Method where CP will be measured at the maximum increase or acceleration of fluorescence, even if the fluorescence levels between curves are different (14).”
Quantitative detection of Citrus tristeza virus in plant tissues and single aphids by real-time RT-PCR (2008, methodology): “To confirm the suitability of the standard curve generated with the transcripts for an accurate quanti- tation of CTV targets, amplification efficiencies of standards and field sample reactions were compared according to Rasmussen (2001) and Olmos et al. (2005).”