Nucleic Acids Research

By G. Blackburn, G. Taylor, G. Thatcher, A. McLennan. 2010

The transcription of chromatin from adenovirus 2 transformed rat cells by murine plasmacytoma RNA polymerases I, II and III has been studied. Both the total RNA synthesis and transcription of the integrated adenovirus 2 genes by RNA polymerase II represent de novo DNA transcription as assessed by their sensitivity to actinomycin D. It is shown that each RNA polymerase class has characteristic ionic strength activation profiles and metal ion requirements. RNA polymerase II transcribes the integrated adenovirus 2 genes in chromatin at a frequency 25to 50-fold higher than their sequences are represented in the genome. In contrast, no detectable viral RNA is synWesized when deproteinized DNA is transcribed. In the presence of Mn , all three RNA polymerases (I,II and III) transcribe the integrated viral enes at approximately the same relative frequency. However, with Mg2+ as divalent cation, the proportion of the total RNA which represents viral gene transcripts is increased 3to 4-fold with RNA polymerase II, while it remains unchanged for RNA polymerases I or II

Citations in the corpus

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