Membrane proteins, lipids and detergents: not just a soap opera.

By A. Seddon, P. Curnow, P. Booth. Biochimica et Biophysica Acta, 2004

1,308 citations recorded by the source corpus.

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

Application of Ni(II)-Assisted Peptide Bond Hydrolysis to Non-Enzymatic Affinity Tag Removal (2012, methodology): “The effects of 1 mM dithiotreitol (DTT), 0.6 M guanidine chloride (GuHCl) and 1% detergents: Triton X100, Tween 20, Thesit, n-dodecyl-b-D-maltoside (DDM), CHAPS were studied with the same fusion protein concentration in 100 mM Hepes buffer, pH 8.2 containing 4 mM Ni(II).”
Microfluidic Devices for Drug Delivery Systems and Drug Screening (2018, background): “The two main types of emulsions are O/W, in which the hydrophilic head of the lipid/surfactant faces the aqueous exterior and the hydrophobic (or lipophilic) tail faces the inner core, and W/O emulsions, in which the hydrophilic head groups face toward the interior phase and the hydrophobic tail gro”
Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins. (2006, background): “However, if the protein is not homogenous or fails to crystallize, then one may vary a number of parameters that might include the detergent and/or lipids used in purification and crystallization (Garavito and Ferguson-Miller, 2001; le Maire et al., 2000; Long et al., 2005; Seddon et al., 2004) and ”