Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins.
By T. Kawate, Eric Gouaux. Structure, 2006
708 citations recorded by the source corpus.
Subfields: Genetics, Materials Chemistry, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/37640e0fa279af0c5900755611b2f614e3b78d72
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Citations in the corpus
- In vitro reconstitution of Wnt acylation reveals structural determinants of substrate recognition by the acyltransferase human Porcupine (2018, methodology): “Protein expression by selected colonies in 1–2-ml scale cultures were assessed using fluorescence-detection size-exclusion chromatography (42).”
- Structure of the class D GPCR Ste2 dimer coupled to two G proteins (2020, positive): “Fluorescence-detection size-exclusion chromatography (FSEC) assays were performed following established procedures 57 .”
- An Overview of the Top Ten Detergents Used for Membrane Protein Crystallization (2017, background): “The screening can be optimized via the introduction of GFP-fusion for a rapid assessment of detergent solubilization efficiency [86] and protein stability in a certain detergent [86,87].”