Molecular Interaction Studies Using Microscale Thermophoresis
By Moran Jerabek-Willemsen, C. Wienken, D. Braun, P. Baaske, S. Duhr. Assay and drug development technologies, 2011
779 citations recorded by the source corpus.
The use of infrared laser sources for creation of localized temperature fields has opened new possibilities for basic research and drug discovery. A recently developed technology, Microscale Thermophoresis (MST), uses this temperature field to perform biomolecular interaction studies. Thermophoresis, the motion of molecules in temperature fields, is very sensitive to changes in size, charge, and solvation shell of a molecule and thus suited for bioanalytics. This review focuses on the theoretical background of MST and gives a detailed overview on various applications to demonstrate the broad applicability. Experiments range from the quantification of the affinity of low-molecular-weight binders using fluorescently labeled proteins, to interactions between macromolecules and multi-component complexes like receptor containing liposomes. Information regarding experiment and experimental setup is based on the Monolith NT.115 instrument (NanoTemper Technologies GmbH).
Subfields: Biophysics, Computational Mechanics, Physical and Theoretical Chemistry
View Paper: https://www.semanticscholar.org/paper/75dc97909c0f0434cfc37c8560b60811d828786f
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
Citations in the corpus
- Twenty years on: the impact of fragments on drug discovery (2016, background): “Ligand binding changes migration behaviour, for example, by altering the charge or hydration shell, and can therefore be detected by microscale thermophoresis 56 .”