High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells

By Yuexin Zhou, Shiyou Zhu, Changzu Cai, Pengfei Yuan, Chunmei Li, Yanyi Huang, Wensheng Wei. Nature, 2014

713 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/73e1287f7675445c910796e0bb9623676beab579

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Citations in the corpus

The new frontier of genome engineering with CRISPR-Cas9 (2014, positive): “A genomescale lentiviral sgRNA library was developed to generate a pooled loss-of-function genetic screening approach suitable for both positive and negative selection (100, 101).”
Quality control, modeling, and visualization of CRISPR screens with MAGeCK-VISPR (2015, background): “Conclusion The recently developed CRISPR screening is a powerful technology in functional studies with different foci, including tumor progression and metastasis [29], drug resistance [3], immune response [30], and stem cell differentiation [4].”
Future directions in breeding for disease resistance in aquaculture species (2017, background): “For viral and bacterial disease resistance traits, genome editing in cell line models (e.g. Zhou et al., 2014) may be an important intermediate step to target and validate putative causative genes.”
CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling (2014, background): “…and large-scale sgRNA libraries for genetic screening (Findlay et al., 2014; Koike-Yusa et al., 2014; Sanjana et al., 2014; Shalem et al., 2014; Wang et al., 2014; Zhou et al., 2014), the Cas9 mouse may be directly combined with genome-wide or targeted sgRNA library to uncover novel biology.”
Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 (2014, neutral): “, [43]), ribosome profiling [44] and CRISPR/Cas-library assays [45].”
WILDS: A Benchmark of in-the-Wild Distribution Shifts (2020, neutral): “, 2019), genomics (Echeverri & Perrimon, 2006; Zhou et al., 2014), proteomics and metabolomics (Taylor et al.”
MAGeCK enables robust identification of essential genes from genome-scale CRISPR/Cas9 knockout screens (2014, background): “These libraries allow both negative and positive selection screening to be conducted on mammalian cell lines in a cost-effective manner [3-6].”