ι-Carrageenases Constitute a Novel Family of Glycoside Hydrolases, Unrelated to That of κ-Carrageenases*
By T. Barbeyron, Gurvan Michel, P. Potin, Bernard Henrissat, B. Kloareg. Journal of Biological Chemistry, 2000
115 citations recorded by the source corpus.
ι-Carrageenases are polysaccharide hydrolases that cleave the β-1,4 linkages between thed-galactose-4-sulfate and 3,6-anhydro-d-galactose-2-sulfate residues in the red algal galactans known as ι-carrageenans. We report here on the purification of ι-carrageenase activity from the marine bacterium Zobellia galactanovorans and on the characterization of ι-carrageenase structural genes. Genomic libraries from this latter bacterium as well as from Alteromonas fortis were functionally screened for the presence of ι-carrageenase+ clones. The Z. galactanovorans and A. fortis ι-carrageenase genes encode homologous proteins of 53.4 and 54.8 kDa, respectively. Based on hydrophobic cluster analysis and on the 1H NMR monitoring of the products of the overexpressed A. fortisι-carrageenase, these enzymes appear to form a new family of glycoside hydrolases, unrelated to that of κ-carrageenases and with an inverting mechanism of hydrolysis. They both feature a 45-amino acid-long N-terminal segment with sequence similarity to the N-terminal region of several other polysaccharidases. In those for which a three-dimensional structure is available, this conspicuous segment, also deemed “glycanase motif” (Chua, J. E. H., Manning, P. A., and Morona, R. (1999) Microbiology(Reading) 145, 1649–1659), corresponds to a strand-helix-strand “cap” that covers the N-terminal end of a common, right-handed β-helical fold.
Subfields: Aquatic Science, Biotechnology, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/73c2dd76b177eda6c7f0f432d59e7e2e93b0c327
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In Aquatic Science
Citations in the corpus
- Chemical Structures and Bioactivities of Sulfated Polysaccharides from Marine Algae (2011, unknown): “microorganisms [83,104–108].”
- Carrageenan: a review. (2018, background): “Lambda type carrageenan has an ester sulfate content of about 32 to 39% and no content of 3,6-AG (Barbeyron et al. 2000).”