A TALE nuclease architecture for efficient genome editing

By Jeffrey C. Miller, Siyuan Tan, Guijuan Qiao, Kyle A. Barlow, Jianbin Wang, Danny F. Xia, Xiangdong Meng, David E. Paschon, Elo Leung, Sarah Hinkley and 10 more. Nature Biotechnology, 2011

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Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases (2014, methodology): “Here, we present a novel algorithm termed Cas-OFFinder that searches for potential offtarget sites in a given genome or user-defined sequences.”
FLASH Assembly of TALENs Enables High-Throughput Genome Editing (2012, positive): “We sought to construct TALE repeat arrays using the same architecture first described by Miller et al. 6 in which four distinct TALE repeat backbones that differ slightly in their amino acid and DNA sequences occur in a repeated pattern.”
Engineering the Delivery System for CRISPR-based Genome Editing (2018, background): “By fusing these proteins to nucleases, it is possible to create a double-strand break at a specified site in the genome, which would then be repaired either through non-homologous end joining, usually leading to a small insertion or deletion and thus gene knockout, or homologous recombination with a”
Genome Engineering of Crops with Designer Nucleases (2012, background): “…a variety of organisms such as Arabidopsis thaliana (Cermak et al., 2011), tobacco (Mahfouz et al., 2011), rice (Li et al., 2012), yeast (Saccharomyces cerevisiae) (Li et al., 2011), zebrai sh (Huang et al., 2011; Sander et al., 2011a), rats (Tesson et al., 2011) and humans (Miller et al., 2011).”