Composition and Three-Dimensional Architecture of the Dengue Virus Replication and Assembly Sites
By Sonja Welsch, S. Miller, I. Romero-Brey, A. Merz, C. Bleck, P. Walther, S. Fuller, C. Antony, J. Krijnse-Locker, Ralf Bartenschlager. Cell Host and Microbe, 2009
1,060 citations recorded by the source corpus.
Positive-strand RNA viruses are known to rearrange cellular membranes to facilitate viral genome replication. The biogenesis and three-dimensional organization of these membranes and the link between replication and virus assembly sites is not fully clear. Using electron microscopy, we find Dengue virus (DENV)-induced vesicles, convoluted membranes, and virus particles to be endoplasmic reticulum (ER)-derived, and we detect double-stranded RNA, a presumed marker of RNA replication, inside virus-induced vesicles. Electron tomography (ET) shows DENV-induced membrane structures to be part of one ER-derived network. Furthermore, ET reveals vesicle pores that could enable release of newly synthesized viral RNA and reveals budding of DENV particles on ER membranes directly apposed to vesicle pores. Thus, DENV modifies ER membrane structure to promote replication and efficient encapsidation of the genome into progeny virus. This architecture of DENV replication and assembly sites could explain the coordination of distinct steps of the flavivirus replication cycle.
Subfields: Infectious Diseases, Public Health, Environmental and Occupational Health
View Paper: https://www.semanticscholar.org/paper/7277eb823c1dbe4abee6f13c9c7ffc95e625a1b4
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Public Health, Environmental and Occupational Health
Citations in the corpus
- Dengue virus induced autophagy regulates lipid metabolism (2010, background): “Cell Host & Microbe 8, 422– on cryo-EM tomography studies (Welsch et al., 2009).”
- Dengue and severe dengue (2025, positive): “Imaging studies of DENV-infected cells have found extensive rearrangements of ER membranes into replication organelles, which facilitate DENV RNA genome synthesis, viral protein translation, and virion assembly (57, 58).”