An evaluation of the fungal 'morphotype' concept based on ribosomal DNA sequences

By D. Lacap, K. D. Hyde, E. Liew. 2003

205 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/7258410e3d19db824e2a1107938bc414552d0ec6

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Citations in the corpus

Molecular characterization of fungal endophytic morphospecies isolated from stems and pods of Theobroma cacao (2006, background): “Others have grouped such isolates into morphospecies (Fröhlich et al., 2000; Guo et al., 2003; Lacap et al., 2003), as was initially done in this study.”
Endophytic fungal diversity: review of traditional and molecular techniques (2012, result): “A number of previous studies have demonstrated that ITS is insufficient for some species delimitation, especially in rapidly evolving or highly diverse genera or species complexes (Lacap et al. 2003; Gazis et al. 2011).”
Morphological and molecular characterization of Colletotrichum species from herbaceous plants in Thailand (2005, unknown): “Lacap et al. (2003) have shown that some morphotypes concur well with species when tested using molecular data.”
Phylogenetic evaluation of species nomenclature of Pestalotiopsis in relation to host association (2004, methodology): “All fungi used in this experiment were cultured on Potato Dextrose Agar and total genomic DNA was extracted following a 2X CTAB protocol as outlined by Jeewon et al. (2003) and Lacap et al. (2003).”
Colletotrichum gloeosporioides is not a common pathogen on tropical fruits (2010, unknown): “Based on similarities in colony characteristics following growth on PDA for 7 days at 27°C, the isolates could be grouped into seven morphotypes (Lacap et al. 2003).”
Phylogenetic evaluation and taxonomic revision of Schizothecium based on ribosomal DNA and protein coding genes (2005, methodology): “Isolates were grown on potato dextrose agar (PDA) for 2-4 weeks and total genomic DNA was extracted from fresh mycelium using the protocol as outlined by Jeewon et al. (2003) and Lacap et al. (2003).”
Diversity of fungal endophytes in leaves and stems of wild rubber trees (Hevea brasiliensis) in Peru (2010, background): “However, since in many fungal lineages ITS sequences present a high interspecific similarity (Lacap et al. 2003; Chaverri & Samuels 2003; Seifert et al. 2007), OTU boundaries were set to 98 % similarity.”
Endophytic fungi associated with lichens in Baihua mountain of Beijing, China (2007, methodology): “Sterile endophytes, which cannot be identified based on the morphological characteristics due to nonsporulation, were placed into different fungal groups using ITS sequence analyses (Guo et al., 2000, 2003; Lacap et al., 2003; Wang et al., 2005; Promputtha et al., 2005).”
Isolation and identification of two flavonoid-producing endophytic fungi from Ginkgo biloba L. (2010, methodology): “Pure fungal cultures were obtained by the methods described by Lacap et al. (2003) and Promputtha et al. (2005).”
Endophytic fungal communities in woody perennials of three tropical forest types of the Western Ghats, southern India (2011, background): “Sterile forms with different culture characteristics were assumed to represent different taxonomic entities (Lacap et al. 2003).”
Endophytic mycobiota of leaves and roots of the grass Holcus lanatus (2010, methodology): “After retaining only one morphologically similar isolate (morphospecies—sensu Lacap et al. 2003) from each plant, 199 isolates from leaves and 149 from roots were obtained and processed for further identification (Table 1).”