Denitrification within the genus Azospirillum and other associative bacteria

By K. Kloos, A. Mergel, C. Rösch, H. Bothe. 2001

216 citations recorded by the source corpus.

Different Azospirillum strains and some other plant growth-promoting rhizobacteria (PGPR) were screened for the occurrence of genes coding for denitrification and nitrogenase reductase (nifH) using polymerase chain reaction (PCR)-based techniques. All PGPR examined were nitrogenasc-positive. Azospirillum strains were remarkably dissimilar with respect to denitrification capabilities, in particular with respect to genes of the dissimilatory nitrite reductase, A. brasilense, A. lipoferum and A. halopraeferens strains possess a cytochrome cd 1 -containing nitritc reductase with low sequence similarities among them. A. irakense and A. doebereinerae have a Cu-containing nitrite reductase and A. amazonense is unable to denitrify. The molecular data were corroborated by activity measurements. The current results indicate that the inability to perform denitrification is unlikely a selective advantage for Azospirillum spp. and other associative bacteria for forming an association with plant roots.

Subfields: Plant Science, Pollution

View Paper: https://www.semanticscholar.org/paper/7159d16268a4e4f9a7c0de10056e9817301ccb23

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Plant Science

1. Y. Bashan
2. L. de-Bashan
3. G. Holguin
4. V. Reis
5. A. Oliveira
6. L. Philippot
7. S. Hallin
8. S. Urquiaga

In Pollution

1. L. Philippot
2. D. Bru
3. S. Hallin
4. Y. Bashan
5. L. de-Bashan
6. J. Chee-Sanford
7. Christopher M. Jones

Citations in the corpus

• Quantitative Detection of the nosZ Gene, Encoding Nitrous Oxide Reductase, and Comparison of the Abundances of 16S rRNA, narG, nirK, and nosZ Genes in Soils (2006, background): “In order to relate with more accuracy the diversity and density data in future studies, quantitative PCR primers were designed in the regions flanking the nosZ fragment amplified for diversity analysis (13).”
• Azospirillum-plant relationships: physiological, molecular, agricultural, and environmental advances (1997-2003). (2004, background): “A. brasilense, A. lipoferum, and A. halopraeferens strains possess cytochrome cd1-containing nitrite reductases with low sequence similarities, while A. irakense and A. doebereinerae have Cu-containing nitrite reductases, and A. amazonense does not denitrify (Kloos et al. 2001).”
• Characterization of bacterial communities in soil and sediment of a created riverine wetland complex using high-throughput 16S rRNA amplicon sequencing (2014, unknown): “…72 ◦C 30 s, 80 ◦C 30 sb; 40 cycles: 95 ◦C 15 s, 61 ◦C 30 s, 72 ◦C 30 s, 80 ◦C 30 sb; 1 cycle: 95 ◦C 15 s, 60 ◦C 30 s, 72 ◦C 10 min Melting curve: 60–95 ◦C nosZF nosZ1622R nosZ 453 Kloos et al. (2001), Throbäck et al. (2004) 1 cycle: 95 ◦C 10 min; 40 cycles: 95 ◦C 15 s; 61.5 ◦C 30 s; 72 ◦C 30 s…”
• Azospirillum amazonense inoculation: effects on growth, yield and N2 fixation of rice (Oryza sativa L.) (2008, background): “Unlike A. brasilense and A. lipoferum species, physiological and genetic studies point out that A. amazonense does not denitrify (Kloos et al. 2001).”
• Reassessing PCR primers targeting nirS, nirK and nosZ genes for community surveys of denitrifying bacteria with DGGE. (2004, background): “[18] developed more pertinent broad range primers for nosZ.”
• Unexpected nondenitrifier nitrous oxide reductase gene diversity and abundance in soils (2012, background): “PCR assays confirmed that commonly used nosZ-targeted primer sets including Nos661F/Nos1773R (52), NosZ-F/nosZ1622R (51, 53), nosZ1F/nosZ1R, and nosZ2F/nosZ2R (49) are not comprehensive and fail to amplify the atypical nosZ genes.”