Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells.
By A. Nocker, C. Cheung, A. Camper. Journal of Microbiological Methods, 2006
1,034 citations recorded by the source corpus.
Subfields: Endocrinology, Molecular Medicine
View Paper: https://www.semanticscholar.org/paper/7045ef1a5a3efa9882dbdd18b92c8001b2c69296
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In Endocrinology
In Molecular Medicine
Citations in the corpus
- Relic DNA is abundant in soil and obscures estimates of soil microbial diversity (2016, methodology): “471 The approach we used was selected because i) it can be used for high-throughput 472 analyses; ii) the utility of PMA for the purposes of distinguishing extracellular DNA from 473 DNA contained within cells is well documented and robust (15, 16); and iii) key 474 assumptions in other methods are ”
- Detection of viable but non-culturable Escherichia coli O157:H7 from vegetable samples using quantitative PCR with propidium monoazide and immunological assays (2013, methodology): “monocytogenes, using pure culture, environmental and food samples (Elizaquivel, Sanchez, & Aznar, 2012; Liang, Dong, Luo, & Li, 2011; Martinon, Cronon, & Wilkinson, 2011; Nocker & Camper, 2006, 2009; Nocker et al., 2006; Nocker, Sossa, & Camper, 2007; Pan & Breidt, 2007; Rudi et al., 2005; Wang, Li,”
- Microbiome definition re-visited: old concepts and new challenges (2020, methodology): “[154] by using popidium monoazide (PMA) which only penetrates membrane-damaged cells, where a photoinduced azide group covalently binds to DNA; this cross-linking effectively inhibits PCR amplification of DNA from dead cells of both Gram-negative and Grampositive bacteria.”
- A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything (2017, background): “There is a preference for PMA over EMA, as it was shown that EMA penetrates the membranes of live bacterial cells (Nocker et al., 2006).”