Roundup Ready® soybean event-specific real-time quantitative PCR assay and estimation of the practical detection and quantification limits in GMO analyses

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Detection and traceability of genetically modified organisms in the food production chain. (2004, methodology): “It is possible to distinguish between three types of detection and quantification limits (Berdal and Holst-Jensen, 2001): (1) the absolute limits, i.e. the lowest number of copies that must be present at the beginning of the first PCR cycle to obtain a probability of at least 95% of…”
Gene Flow, Risk Assessment and the Environmental Release of Transgenic Plants (2008, background): “The cost of these techniques and the occurrence of false positives is however a potential problem when looking for very rare events (Begg et al., 2007; Berdal and Holst-Jensen, 2001).”