Genome editing in rice and wheat using the CRISPR/Cas system

By Qi-wei Shan, Yanpeng Wang, Jun Li, Caixia Gao. Nature Protocols, 2014

660 citations recorded by the source corpus.

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation (2015, neutral): “system provides an alternative method [17–44].”
Plant Innate Immune Response: Qualitative and Quantitative Resistance (2016, background): “based on agro-transformation or direct introduction to the protoplast (Shan et al., 2014).”
Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA (2016, positive): “We analysed the sgRNA target site in the regenerated T0 seedlings using PCR restriction enzyme digestion assay (PCR-RE assay) 8 , ﬁrst using a conserved primer set (Supplementary Table 2) that recognizes all three TaGASR7 homoeologues and then with three primer pairs speciﬁc for each homoeologue…”
Enhanced Rice Blast Resistance by CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922 (2016, background): “In particular, CRISPR/Cas9 has been demonstrated to be the most effective SSN thus far and has been used for genome editing in major crops such as rice [29–41], maize [42–45], wheat [39, 46, 47], sorghum [30], tomato [48, 49], soybean [50–52] and potato [53].”