Metabolic engineering of an alternative pathway for ascorbic acid biosynthesis in plants

By Ashok K. Jain, C. Nessler. Molecular breeding, 2000

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Molecular genetic approaches to increasing mineral availability and vitamin content of cereals (2007, background): “Transformation of tobacco and lettuce with a rat cDNA encoding L-gulono-g-lactone oxidase resulted in an increase in the vitamin C content of up to seven-fold (Jain and Nessler, 2000).”
Over-expression of strawberry d-galacturonic acid reductase in potato leads to accumulation of vitamin C with enhanced abiotic stress tolerance (2009, background): “However, in animals the terminal step of L-gulono-1,4-lactone conversion to AsA is catalyzed by L-gulono-1,4-lactone oxidase (GUO) enzyme [9].”
Biosynthesis and Catabolism of L-Ascorbic Acid in Plants (2005, background): “Alternatively, the enhanced AsA levels in transgenic plants overexpressing rat L-GulLO (Jain and Nessler, 2000; Radzio et al., 2003) may be due to the fact that the enzyme reacts with L-GalL in vivo as the relative activity of the enzyme for this substrate is approximately 70 percent of that with L-”