Quantitative analysis of prey DNA in pinniped faeces: potential to estimate diet composition?

By B. Deagle, D. Tollit. Conservation Genetics, 2007

163 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/6d7dc8bd8b55b371678d25e7b58c300a1ed1d10e

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Ecology

In Molecular Biology

Citations in the corpus

Who is eating what: diet assessment using next generation sequencing (2012, background): “Many studies have examined DNA-based approaches for studying diet with captive animals (e.g. Hoogendoorn & Heimpel 2001; Deagle et al. 2005; Foltan et al. 2005; Weber & Lundgren 2009) and a few have specifically focused on Quantitative aspects (Deagle & Tollit 2007; Bowles et al. 2011).”
Methods of estimating marine mammal diets: A review of validation experiments and sources of bias and uncertainty (2012, methodology): “Real-time PCR has also been shown to successfully amplify prey DNA without amplifying DNA from the predator (Deagle and Tollit 2007).”
Diet studies of seabirds: a review and recommendations (2007, background): “Deagle and Tollit (2007) analysed faeces from captive sea lions fed a diet containing three fish species (50%, 36%, and 14% by weight) using real-time PCR to quantify mtDNA in undigested tissue and in the faecal samples.”
The trophodynamics of marine top predators: Current knowledge, recent advances and challenges (2015, background): “Historically, the majority of diet information for marine mammals have come from stranded individuals, stable isotope analysis or scat in the case of pinnipeds (Deagle and Tollit, 2007; Hobson et al., 1996; Pauly et al., 1998).”
Use of DNA metabarcoding for stomach content analysis in the invasive lionfish Pterois volitans in Puerto Rico (2016, background): “However, the underlying variability in DNA quality, differential breakdown of that DNA during digestion, and differences in digestion stages (Deagle & Tollit 2007, Troedsson et al. 2009, Valentini et al. 2009b), as well as the objective of identifying several different organisms within the same…”
Counting with DNA in metabarcoding studies: How should we convert sequence reads to dietary data? (2018, background): “Detailed captive feeding studies examining quantitative prey DNA recovery have been carried out on captive seals and sea lions (Bowles et al. 2011; Deagle & Tollit 2007; Thomas et al. 2014).”
Pyrosequencing faecal DNA to determine diet of little penguins: is what goes in what comes out? (2010, unknown): “However, correction factors derived from information on mtDNA density of the fish (using formula in Deagle and Tollit 2007) do not improve our estimates of diet composition from the penguin faeces.”