Multiplex PCR Strategy for Rapid Identification of Structural Types and Variants of the mec Element in Methicillin-Resistant Staphylococcus aureus

By Duarte C. Oliveira, Hermínia de Lencastre. Antimicrobial Agents and Chemotherapy, 2002

1,430 citations recorded by the source corpus.

Subfields: Infectious Diseases, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/6d0a3e42c292d75a05537456c15438caff1aa8b5

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Citations in the corpus

Combination of Multiplex PCRs for Staphylococcal Cassette Chromosome mec Type Assignment: Rapid Identification System for mec, ccr, and Major Differences in Junkyard Regions (2006, background): “Both Oliveira and Lencastre and Zhang et al. happened to design primers on the nucleotide sequence of SCCmercury for the identification of type III SCCmec elements.”
Severe community-onset pneumonia in healthy adults caused by methicillin-resistant Staphylococcus aureus carrying the Panton-Valentine leukocidin genes. (2005, methodology): “performed by multiplex PCR strategy [19], with the modifi-”
Novel Multiplex PCR Assay for Characterization and Concomitant Subtyping of Staphylococcal Cassette Chromosome mec Types I to V in Methicillin-Resistant Staphylococcus aureus (2005, methodology): “The only previously described multiplex PCR assay for SCCmec typing (24) is more difficult to interpret and is limited in its ability to detect SCCmec subtypes IVa, b, c, and d plus the newly described type V, these groups being implicated in currently emerging community MRSA outbreaks (12, 31).”
The evolution of Staphylococcus aureus. (2008, methodology): “1) (Ito et al., 2001, 2003; Leclercq, 2002; Oliveira et al., 2006a; Takano et al., 2008).”
Methicillin-Resistant Staphylococcus aureus: Molecular Characterization, Evolution, and Epidemiology (2018, background): “developed by Oliveira and de Lencastre (321) in 2002.”
A Field Guide to Pandemic, Epidemic and Sporadic Clones of Methicillin-Resistant Staphylococcus aureus (2011, methodology): “All Irish MRSA isolates underwent SCCmec typing for (i) the ccr and mec complex genes and (ii) the J regions and mecI [15;85;246;247].”
Typing of Methicillin-Resistant Staphylococcus aureus in a University Hospital Setting by Using Novel Software for spa Repeat Determination and Database Management (2003, background): “A more detailed picture of the microevolution of clonal complex 5 isolates circulating at the hospital might be achieved, e.g., by determination of SCCmec types (8, 22).”
Emergence of community-associated methicillin-resistant Staphylococcus aureus USA300 genotype as a major cause of health care-associated blood stream infections. (2006, methodology): “SCCmec genotyping of MRSA isolates was performed using a multiplex PCR method as described elsewhere [30].”
The molecular evolution of methicillin-resistant Staphylococcus aureus. (2007, background): “a Locus A (495 bp) is located downstream of the pls gene; locus B (284 bp) is internal to the kdp operon; locus C (209 bp) is internal to the mecI gene; locus D (342 bp) is internal to the dcs region; locus E (243 bp) is located in the region between integrated plasmid pI258 and transposon Tn554; an”