Novel Multiplex PCR Assay for Characterization and Concomitant Subtyping of Staphylococcal Cassette Chromosome mec Types I to V in Methicillin-Resistant Staphylococcus aureus

By Kunyan Zhang, J. McClure, S. Elsayed, T. Louie, J. Conly. Journal of Clinical Microbiology, 2005

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Methicillin-Resistant Staphylococcus aureus: Molecular Characterization, Evolution, and Epidemiology (2018, neutral): “In 2005, Zhang and colleagues (322) developed”
A Field Guide to Pandemic, Epidemic and Sporadic Clones of Methicillin-Resistant Staphylococcus aureus (2011, background): “This strain has a characteristic variant of a SCCmec V element, in which two ccrC genes are present (SCCmec VT or 5C2&5, GenBank AB12129, [132]).”
Combination of Multiplex PCRs for Staphylococcal Cassette Chromosome mec Type Assignment: Rapid Identification System for mec, ccr, and Major Differences in Junkyard Regions (2006, background): “Our M-PCRs do not conflict with two previously reported M-PCRs based primarily on the identification of junkyard regions (20, 26).”
The molecular evolution of methicillin-resistant Staphylococcus aureus. (2007, background): “[41] developed a multiplex PCR for the characterisation of SCCmec types I–V.”
The evolution of Staphylococcus aureus. (2008, methodology): “1) (Ito et al., 2001, 2003; Leclercq, 2002; Oliveira et al., 2006a; Takano et al., 2008).”