An optimized transgenesis system for Drosophila using germ-line-specific φC31 integrases

By J. Bischof, R. Maeda, M. Hediger, F. Karch, Konrad Basler. Proceedings of the National Academy of Sciences of the United States of America, 2007

1,931 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/6c98d232025b7658a65e30fde5d70d7b0f93ca8e

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Citations in the corpus

Genetic dissection of neural circuits. (2008, neutral): “, 2003) can fire action potentials with short latencies and small response jitter in response to illumination with blue light (Boyden et al., 2005; Li et al., 2005; Zhang and Oertner, 2006).”
Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila (2014, methodology): “These plasmids were integrated into the Drosophila genome at defined positions using the attB/attP/Phi31C system (18, 19).”
Highly Specific and Efficient CRISPR/Cas9-Catalyzed Homology-Directed Repair in Drosophila (2014, background): “Germline expression of Cas9 increases targeting efficiency Previous work has shown that germline expression of FC31 phage recombinase yields increased transformation efficiency over injection protocols (Bischof et al. 2007).”
Origins of Cell-Type-Specific Olfactory Processing in the Drosophila Mushroom Body Circuit. (2017, neutral): “…Center RRID: BDSC_42749 Drosophila, UAS-Archaerhodopsin-3::GFP in attP40 This paper N/A Recombinant DNA FCK-Arch-GFP Addgene, Chow et al., 2010 22217 pUASTattB Gift from A.W. Moore, Bischof et al., 2007 FlyBase ID: FBmc0003002 (Continued on next page) e1 Neuron 95, 357–367.e1–e4, July 19, 2017”
Genetic manipulation of genes and cells in the nervous system of the fruit fly (2011, methodology): “These plasmids were then integrated at a specific docking site in the fly genome using the FC31 integrase (Groth et al., 2004; Bischof et al., 2007) and tested for expression, resulting in thousands of GAL4 lines (Pfeiffer et al., 2008).”
The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing and Mosaic Analysis (2010, neutral): “…pQF-M2ET was PCR amplified to include EcoRI/NotI restriction sites and inserted into the pattB vector (Bischof et al., 2007). pattB-QF-hsp70 The QF-hsp70 cassette from pBac-GH146-QF was PCR amplified to include EcoRI/NotI restriction sites and inserted into the pattB vector (Bischof et al., 2007).”
A census of human RNA-binding proteins (2014, methodology): “, and using the phiC31 integrase transgenesis system (Bischof et al., 2007), and site-specifically integrated to generate transgenic flies at the 25C7 landing site in”
Variant ionotropic glutamate receptors as chemosensory receptors in Drosophila. (2009, methodology): “Transgenic lines were generated in this study by Genetic Services Inc. (Cambridge, MA) with either P-element-mediated transformation or, for UAS-IR84a, the phiC31-based integration system (Bischof et al., 2007).”
Ancient Protostome Origin of Chemosensory Ionotropic Glutamate Receptors and the Evolution of Insect Taste and Olfaction (2010, methodology): “). All IR promoter-GAL4 constructs were inserted in the same genomic location using the phiC31 integrase system”
Complementary Function and Integrated Wiring of the Evolutionarily Distinct Drosophila Olfactory Subsystems (2011, methodology): “6 coding sequence (Ohkura et al., 2005) with gypsy insulator sequences (Markstein et al., 2008) in pUAST attB (Bischof et al., 2007).”