Photoassimilate allocation and dynamics of hotspots in roots visualized by 14C phosphor imaging

By J. Pausch, Y. Kuzyakov. 2011

87 citations recorded by the source corpus.

Understanding photoassimilate allocation into the roots and the release of organic substances from the roots into the rhizosphere is an important prerequisite for characterizing the belowground C input, the spatial and temporal distribution of C, and the interactions between plants and soil microorganisms. Based on 14C phosphor imaging, we visualized the allocation of assimilates into Lolium perenne roots and estimated the life time of hotspots at the root tips. Lolium shoots were labeled in a 14CO2 atmosphere, and herbariums of roots and shoots were prepared 6 h, 2 d, and 11 d after the 14C pulse. The 14C distribution in roots and leaves revealed that pulse labeling does not yield homogeneously labeled plant material. The spatial distribution of assimilate allocation was evaluated based on the 14C specific activity expressed as digital light units (DLUmm–2) of the imaging plates. Areas with high relative 14C activity were classified as hotspots. Strong 14C hotspots were detected mainly at the root tips already 6 h after the 14C assimilation, and they remained active for at least 2 d. Eleven days after the 14C assimilation, the hotspots at the root tips disappeared and the 14C distribution was much more even than after 6 h or after 2 d. 14C phosphor imaging proved to be a promising tool to visualize the allocation of photoassimilates into the roots and the rhizosphere and can be used to identify hotspots and their dynamics.

Subfields: Plant Science, Pollution, Soil Science

View Paper: https://www.semanticscholar.org/paper/69a8a3665584a140847ad508b1795206575befe2

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Plant Science

1. Y. Kuzyakov
2. J. Pausch
3. E. Blagodatskaya
4. M. Spohn
5. M. Dippold
6. B. Razavi
7. W. Cheng
8. Petr Baldrian

In Pollution

1. Y. Kuzyakov
2. E. Blagodatskaya
3. M. Spohn
4. L. Gianfreda

In Soil Science

1. Y. Kuzyakov
2. J. Pausch
3. E. Blagodatskaya
4. M. Dippold
5. M. Spohn
6. B. Razavi
7. W. Cheng
8. A. Gunina

Citations in the corpus

• Distribution of microbial- and root-derived phosphatase activities in the rhizosphere depending on P availability and C allocation – Coupling soil zymography with 14C imaging (2013, background): “14C imaging (which is also called autoradiography) is a semi-quantitative method, in which the radioactivity of the 14C is transformed into light flashes that can be seen on a light-sensitive screen (Pausch and Kuzyakov, 2011; Wichern et al., 2011).”
• Microbial response to rhizodeposition depending on water regimes in paddy soils (2013, background): “Further, new assimilates are allocated primarily to root tips and increased exudationwas correlated with increasing number of root tips (Thornton et al., 2004; Pausch and Kuzyakov, 2011; Wichern et al., 2011).”
• Carbon input by roots into the soil: Quantification of rhizodeposition from root to ecosystem scale (2018, background): “Grasses have short time lags of only 12.5 hr between photosynthesis and CO2 release from the soil (Kuzyakov & Gavrichkova, 2010; Pausch & Kuzyakov, 2011).”
• Microbial hotspots and hot moments in soil: Concept & review (2015, background): “4) (Pausch and Kuzyakov, 2011).”