CRISPR/Cas, the Immune System of Bacteria and Archaea

By Philippe Horvath, Rodolphe Barrangou. Science, 2010

2,411 citations recorded by the source corpus.

Subfields: Ecology, Epidemiology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/68e23e8e0dc19983b1f81b4be706587d0406ce36

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Citations in the corpus

Insights from 20 years of bacterial genome sequencing (2015, background): “These newer technologies hold the promise of not only generating more sequence for less money, but they may eventually eliminate the concept of draft microbial genomes all together.”
The potential for the use of gene drives for pest control in New Zealand: a perspective (2018, background): “The system consists of a nuclease, Cas9, that can be targeted to any sequence in the genome using a small RNA sequence called a guide RNA (gRNA), providing that target sequence sits next to a 2–6 base pair PAM motif (Horvath & Barrangou 2010).”
One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering (2013, neutral): “Bacteria and archaea have evolved an RNA-based adaptive immune system that uses CRISPR (clustered regularly interspaced short palindromic repeat) and Cas (CRISPR-associated) proteins to detect and destroy invading viruses and plasmids (Horvath and Barrangou, 2010; Wiedenheft et al., 2012).”
Genome Engineering Using the CRISPR Cas9 System (2019, background): “The second stage which is the recognition stage occurs when the phage attack or inject it’s DNA for the second time into the host cell (Bacteria or Archaea), the host CRISPR loci transcribed it spacers into small form of non-coding RNA known as Pre-CRISPR RNA which link up through base pairing with ”
RNA-Guided Human Genome Engineering via Cas9 (2013, neutral): “thermophilus Type II systems require NGGNG (30) and NNAGAAW (31), respectively, while different S.”
CRISPR/Cas system and its role in phage-bacteria interactions. (2010, background): “They are also considered a second marker for CRISPR/Cas systems (43).”
A CRISPR/Cas9 toolkit for multiplex genome editing in plants (2014, background): “This system is based on the bacterial and archaeal clustered regularly interspaced short palindromic repeats (CRISPR) adaptive immune system for purging invading viral and plasmid DNA, which relies on the endonuclease activity of CRISPR-associated (Cas) proteins, with sequence specificity directed b”
Propionibacterium acnes strain populations in the human skin microbiome associated with acne (2013, background): “The CRISPR locus encoded in P. acnes consists of a series of cas genes - cas3, cse1, cse2, cse4, cas5e, cse3, cas1 and cas2, which are homologous to the CRISPR locus reported in E. coli (Figure S10) and the CRISPR4 locus in Streptococcus thermophilus (Horvath and Barrangou, 2010).”