Production of recombinant proteins by microbes and higher organisms.

By A. Demain, Preeti Vaishnav. Biotechnology Advances, 2009

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Hormonal manipulations for the enhancement of sperm production in cultured fish and evaluation of sperm quality (2017, background): “Incorrect glycosylation reduces in vivo half-life and biological activity/receptor binding of the reGtH and for these reasons mammalian cells are the most popular for producing recombinant mammalian glycosylated proteins (Demain and Vaishnav, 2009).”
Prebiotics: Definition, Types, Sources, Mechanisms, and Clinical Applications (2019, background): “But this bacterium has also some disadvantages, including producing proteases in high quantities (which are able to degrade proteins) and plasmid instability [86,87].”
Recombinant protein expression in Escherichia coli: advances and challenges (2014, background): “All have strengths and weaknesses and their choice may be subject to the protein of interest (Demain and Vaishnav, 2009; Adrio and Demain, 2010).”
Microbial Enzymes: Tools for Biotechnological Processes (2014, background): “Dashtban, M.; Qin, W. Overexpression of an exotic thermotolerant β-glucosidase in Trichoderma reesei and its significant increase in cellulolytic activity and saccharification of barley straw.”