Recombinant protein expression in Escherichia coli: advances and challenges
By Germán L. Rosano, E. Ceccarelli. Frontiers in Microbiology, 2014
2,399 citations recorded by the source corpus.
Escherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of heterologous proteins, such as a vast catalog of expression plasmids, a great number of engineered strains and many cultivation strategies. We review the different approaches for the synthesis of recombinant proteins in E. coli and discuss recent progress in this ever-growing field.
Subfields: Molecular Biology, Radiology, Nuclear Medicine and Imaging
View Paper: https://www.semanticscholar.org/paper/a5894597246af57ddc43e476b7ca18041dc6dcbe
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Citations in the corpus
- Expanding functional protein sequence spaces using generative adversarial networks (2021, background): “Indeed, the correct protein folding determines its solubility and is among the main bottlenecks in recombinant protein production, which, apart from the sequence, is highly dependent on experimental conditions 45 .”