Reconstitution of Circadian Oscillation of Cyanobacterial KaiC Phosphorylation in Vitro

By M. Nakajima, K. Imai, Hiroshi Ito, T. Nishiwaki, Yoriko Murayama, H. Iwasaki, T. Oyama, T. Kondo. Science, 2005

1,122 citations recorded by the source corpus.

Subfields: Endocrine and Autonomic Systems, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/67a0b846f759656e200d0470b82e2af25d3a392a

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Citations in the corpus

The Legionella pneumophila kai operon is implicated in stress response and confers fitness in competitive environments (2013, neutral): “In brief, 1 μg of KaiC was incubated for 1 h at 30°C in 10 μl of incubation buffer (20 mM Tris pH 8, 150 mM NaCl, 5 mM MgCl2) containing 5 μCi of [γ-32P]-ATP.”
Circadian rhythms from multiple oscillators: lessons from diverse organisms (2005, background): “Recent data show that a temperature-compensated circadian rhythm of KaiC phosphorylation can be achieved in vitro , with only KaiA, KaiB, KaiC and ATP as components of the cloc”
Suprachiasmatic Nucleus: Cell Autonomy and Network Properties (2010, background): “On the other hand, investigators have recently questioned the view of a mammalian clock based exclusively on transcriptional mechanisms (22), especially in light of the dramatic demonstration that cyanobacterial circadian clock function can be reconstituted in a cell-free system with only three prot”
The mammalian circadian timing system: organization and coordination of central and peripheral clocks. (2010, background): “Moreover, this group succeeded in reconstituting a circadian oscillator in the test tube with just three clock proteins (KaiA, KaiB, and KaiC) and ATP (219).”
Molecular components of the mammalian circadian clock. (2006, background): “cycles fulfilling other necessary attributes of true circadian clocks (Nakajima et al. 2005; O’Neill and Reddy 2011; Tomita et al. 2005).”
Post-translational modifications regulate the ticking of the circadian clock (2007, positive): “In fact, the cyanobacterial circadian clock can be reconstituted in vitro with one autophosphorylating and dephosphorylating protein, KaiC, and two associated regulators, KaiA and Kai”
Stochastic gene expression and its consequences (2008, background): “Notably , the proteins involved can oscillate in vitro in the absence of any transcriptional regulation at all (Nakajima et al., 2005), but presumably variability in the numbers of these proteins in individual cells can cause cells to lose synchrony.”
Modeling the cell cycle: why do certain circuits oscillate? (2011, positive): “Nevertheless, in cyanobacteria (Nakajima et al., 2005; Rust et al., 2007; Tomita et al., 2005), Ostreococcus (O’Neill et al.”
Programming an in vitro DNA oscillator using a molecular networking strategy (2011, positive): “Taking them as a prototypical example of biological RNs, we noticed that their architecture relies mostly on a limited set of basic events: activation, inhibition and destruction.”