OpenCFU, a New Free and Open-Source Software to Count Cell Colonies and Other Circular Objects

By Quentin Geissmann. PLoS ONE, 2012

365 citations recorded by the source corpus.

Counting circular objects such as cell colonies is an important source of information for biologists. Although this task is often time-consuming and subjective, it is still predominantly performed manually. The aim of the present work is to provide a new tool to enumerate circular objects from digital pictures and video streams. Here, I demonstrate that the created program, OpenCFU, is very robust, accurate and fast. In addition, it provides control over the processing parameters and is implemented in an intuitive and modern interface. OpenCFU is a cross-platform and open-source software freely available at http://opencfu.sourceforge.net.

Subfields: Artificial Intelligence, Biophysics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/6729882532abb249a36d7c9b409bf9343f8b7a2b

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Citations in the corpus

Discovery and functional characterization of diverse Class 2 CRISPR-Cas systems (2015, methodology): “After 18 hr, colonies were counted with OpenCFU (Geissmann 2013).”
ColonyArea: An ImageJ Plugin to Automatically Quantify Colony Formation in Clonogenic Assays (2014, methodology): “Alternatively, free distribution software can be employed, either as standalone solutions (CellProfiler, [12] and OpenCFU, [13]) or as macros for ImageJ (National Institutes of Health, Bethesda MD – USA) [4].”