SOX9 is an intestine crypt transcription factor, is regulated by the Wnt pathway, and represses the CDX2 and MUC2 genes

By P. Blache, M. van de Wetering, I. Duluc, C. Domon, P. Berta, J. Freund, Hans Clevers, P. Jay. Journal of Cell Biology, 2004

526 citations recorded by the source corpus.

TCF and SOX proteins belong to the high mobility group box transcription factor family. Whereas TCFs, the transcriptional effectors of the Wnt pathway, have been widely implicated in the development, homeostasis and disease of the intestine epithelium, little is known about the function of the SOX proteins in this tissue. Here, we identified SOX9 in a SOX expression screening in the mouse fetal intestine. We report that the SOX9 protein is expressed in the intestinal epithelium in a pattern characteristic of Wnt targets. We provide in vitro and in vivo evidence that a bipartite β-catenin/TCF4 transcription factor, the effector of the Wnt signaling pathway, is required for SOX9 expression in epithelial cells. Finally, in colon epithelium-derived cells, SOX9 transcriptionally represses the CDX2 and MUC2 genes, normally expressed in the mature villus cells of the intestinal epithelium, and may therefore contribute to the Wnt-dependent maintenance of a progenitor cell phenotype.

Subfields: Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/664d219dce69db1f62e3f097c7677ade881dd29e

How researchers see this paper

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In Genetics

1. Hans Clevers
2. Nick Barker
3. H. Begthel
4. Laurens G. van der Flier
5. M. van de Wetering
6. Maaike van den Born
7. Johan H. van Es
8. R. Nusse

In Molecular Biology

1. Hans Clevers
2. Nick Barker
3. M. van de Wetering
4. H. Begthel
5. Laurens G. van der Flier
6. Maaike van den Born
7. P. Peters
8. D. Stange

Citations in the corpus

• Pancreas organogenesis: From bud to plexus to gland (2011, background): “This role is similar to its function in the hair bulge, intestinal epithelium, and neural crest (Cheung and Briscoe, 2003; Blache et al., 2004; Vidal et al., 2005).”
• Transcription factor achaete scute-like 2 controls intestinal stem cell fate. (2009, background): “As an example, Figure 1B shows the expression of Sox9, a Wnt-responsive gene (Blache et al., 2004) crucial for Paneth cell specification (Bastide et al.”