Chitosan kills Escherichia coli through damage to be of cell membrane mechanism

By Xiao-fang Li, Xiaorong Feng, Sheng Yang, Guo Fu, Ting-pu Wang, Zhong-xing Su. 2010

210 citations recorded by the source corpus.

This paper aims to identify the interaction of chitosan (CTS) with bacterial membrane and delineate the inhibition mechanism of CTS towards Gram-negative bacteria. The inhibition activity of CTS against Escherichia coli (E coli) was evaluated by measuring O.D(610nm). CTS have increased the permeabilities of the outer membrane (OM) and inner membrane (IM) of E. coli, and ultimately disrupted the cell membrane with the release of cellular contents. Morphologies of bacteria treated with CTS were observed by Transmission electron microscopy (TEM), which indicated that it was the primary inhibition action for CTS to damage the bacterial cell membranes. The interaction between CTS and a model phospholipids membrane was studied, and the precipitates formed with CTS and phosphatidylcholine (PC) suggested the damage of bacterial cell membranes was likely to be caused by the electrostatic interaction between -NH(3)(+) groups of CTS and carbonyl and phosphoryl groups of the phospholipid components of the cell membrane. (C) 2009 Published by Elsevier Ltd.

Subfields: Biomaterials, Organic Chemistry, Pharmaceutical Science

View Paper: https://www.semanticscholar.org/paper/659c380fa0e052bbb19167cfcebc7bd081f59c27

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In Biomaterials

1. Sergey Dobretsov
2. S. Jafari
3. M. Hosseinnejad
4. Joydeep Dutta
5. Fei Ye
6. Santosh Kumar

In Organic Chemistry

1. S. Jafari
2. Joydeep Dutta

Citations in the corpus

• Chitosan Nanocomposite Coatings for Food, Paints, and Water Treatment Applications (2019, methodology): “coli (a) treated with buffer and (b–d) chitosan of 50 kDa molecul r weight, (Reproduced with permission fro [61], Copyright© 2010, Elsevier).”
• Physicochemical, functional, antioxidant and antibacterial properties of chitosan extracted from shrimp wastes by microwave technique (2013, methodology): “The antimicrobial activity of natural antimicrobial agents against both gram-positive and gram-negative bacteria strains were examined by detecting of the minimum inhibitory concentrations (MICs), which defined as ‘‘the lowest concentration required for complete inhibition of test organism after inc”
• Chitosan as a Wound Dressing Starting Material: Antimicrobial Properties and Mode of Action (2019, background): “Thus, while HMW chitosan forms a dense layer on the surface of the cell, preventing nutrient uptake [89], the much smaller chitosan molecules seem to penetrate the membrane and even bind to DNA and RNA [98,159].”
• Modification of Chitosan for the Generation of Functional Derivatives (2019, background): “, the physiological state of the microbes, and the nature of the species present, are also key elements modulating microbial sensitivity to CS [100,101,126,140].”
• Evaluation of different factors affecting antimicrobial properties of chitosan. (2016, background): “b the interaction of diffused hydrolysis products with microbial DNA, which leads to the inhibition of the mRNA and protein synthesis [104,10,19,21,77]; c chitosan also inhibits the microbial growth by the chelation of nutrients and essential metals[104,11,50,51,16]; d chitosan on the surface of the”