Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method.

By R. Gietz, R. A. Woods. Methods in Enzymology, 2002

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Prediction of Drug-Target Interactions and Drug Repositioning via Network-Based Inference (2012, methodology): “To evaluate the agonistic or antagonistic activities of the compounds on ER, a yeast two-hybrid system was constructed by yeast co-transformation with pGBKT7-ERa/bLBD and pGADT7-SRC1 according to the lithium acetate method [53].”
TT2, TT8, and TTG1 synergistically specify the expression of BANYULS and proanthocyanidin biosynthesis in Arabidopsis thaliana. (2004, methodology): “Yeast was co-transformed using the AcLi/SSDNA/ PEG method (Gietz and Woods, 2002) and transformants were selected on appropriate media.”
Mapping pathways and phenotypes by systematic gene overexpression. (2006, methodology): “Miniprep DNA was introduced into strain Y4741 by using a high-throughput adaptation of a lithium acetate transformation method (Gietz and Woods, 2002).”
Quantitative proteomics reveals the function of unconventional ubiquitin chains in proteasomal degradation. (2009, positive): “In addition, all plasmids were introduced into the strains by transformation according to the standard LiOAc method ( Gietz and Woods, 2002 ).”