Effective expression of small interfering RNA in human cells

By C. P. Paul, P. Good, I. Winer, D. Engelke. Nature Biotechnology, 2002

844 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/637089d59574bbd6d7274bcb5ee7361cf2e705b6

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Citations in the corpus

CRISPR interference (CRISPRi) for sequence-specific control of gene expression (2013, background): “…sequence does not contain certain sequences, such as restriction enzyme sites for cloning (e.g., EcoRI, BglII and BamHI for bacterial sgRNAs; BstXI and XhoI for human sgRNAs) or transcription terminator sequences (e.g., more than four consecutive Us is a strong terminator for the U6 promoter 54 ).”
Antisense technologies. Improvement through novel chemical modifications. (2003, methodology): “Short hairpin RNA was transcribed using RNA polymerase III promotors that normally control the transcription of either the small nuclear RNA U6 [118,119,121,122] or the H1 RNA component of RNase P [123].”
Interactions between Hyaluronan and Its Receptors (CD44, RHAMM) Regulate the Activities of Inflammation and Cancer (2015, methodology): “Thus, the conditional suppression of gene expression by the use of a CD44vshRNA expressing plasmid holds potential promise for therapeutic approaches for silencing HA-CD44variant signaling and downstream signaling pathways that promote disease causing genes (386).”
RNA silencing: Methods and protocols (2006, methodology): “Two opposing copies of the target sequence are inserted on either side of a “stuffer” DNA, which is required for stability of the plasmid in Escherichia coli, and then the stem loop is cloned into a vector containing an inducible RNA polymerase I promoter (23,24).”
Mouse ES cells express endogenous shRNAs, siRNAs, and other Microprocessor-independent, Dicer-dependent small RNAs. (2008, background): “Exogenous siRNAs are often introduced using vectors expressing shRNAs driven from a pol III promoter (Lee et al. 2002; Paul et al. 2002).”
Lentivirus-delivered stable gene silencing by RNAi in primary cells. (2003, background): “Several recently developed (Brummelkamp et al. 2002a; McCaffrey et al. 2002; McManus et al. 2002; Paddison et al. 2002; Paul et al. 2002; Sui et al. 2002; Xia 2002; Yu et al. 7These authors contributed equally to this work.”
A large-scale RNAi screen in human cells identifies new components of the p53 pathway (2004, neutral): “The oligonucleotides specifying the shRNAs were annealed and cloned in a high-throughput fashion into pRetroSu-per (pRS), a retroviral vector that contains the shRNA expression cassette 9 .”