Isolation and characterization of mineral phosphate-solubilizing bacteria naturally colonizing a limonitic crust in the south-eastern Venezuelan region

By E. Pérez, M. Sulbarán, M. Ball, L. Yarzábal. 2007

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Abstract With the aim to explore the possible role of mineral phosphate-solubilizing bacteria (PSB) in phosphorus (P) cycling in iron-rich, acidic soils, we conducted a survey of PSB naturally colonizing a limonitic crust in the south-east region of Venezuela (Bolivar State). A total of 130 heterotrophic bacterial isolates showing different degrees of mineral tri-calcium phosphate (Ca 3 (PO 4 ) 2 )-solubilizing activities were isolated from NBRIP plates. In contrast, no isolates showing iron phosphate (FePO 4 )- or aluminum phosphate (AlPO 4 )-solubilizing activities were detected by this experimental approach. The 10 best Ca 3 (PO 4 ) 2 -solubilizers were selected for further characterization. These isolates were shown to belong to the genera Burkholderia , Serratia , Ralstonia and Pantoea by partial sequencing analysis of their respective 16S rRNA genes. All the PSB isolates were able to mediate almost complete solubilization of Ca 3 (PO 4 ) 2 in liquid cultures; in contrast, the PSB isolates were less effective when solubilizing FePO 4 . Two groups of PSB isolates were clearly differentiated on the basis of their Ca 3 (PO 4 ) 2 solubilization kinetics. Acidification of culture supernatants seemed to be the main mechanism for P solubilization. Indeed, gluconic acid was shown to be present in the supernatant of five isolates. Furthermore, detection of genes involved in the production of this organic acid was possible in three isolates by means of a PCR protocol.

Subfields: Environmental Chemistry, Environmental Engineering, Pollution

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Citations in the corpus

Isolation and characterization of phosphate-solubilizing bacteria from aerobic rice (2012, result): “Perez et al. (2007) found similar findings that NBRIP media plates showed positive for P solubilizing activity, however, PDYA-AlP plates showed negative results.”
Isolation of culturable phosphobacteria with both phytate-mineralization and phosphate-solubilization activity from the rhizosphere of plants grown in a volcanic soil (2008, background): “Recently, by using specific primer sets and PCR amplification, the presence of two genes involved in P solubilization (gdh and pqqE) was detected in PSB, but three of these strains did not show amplification with any primer sets ( Perez et al. 2007 ).”
Comparison of in vitro solubilization activity of diverse phosphate-solubilizing bacteria native to acid soil and their ability to promote Phaseolus vulgaris growth (2010, neutral): “However, the information available on the occurrence of PSB in acid soils is limited (Pal 1998; Pérez et al. 2007).”
Solubilization of inorganic phosphate and production of organic acids by bacteria isolated from a Moroccan mineral phosphate deposit (2013, background): “This consolidates the hypothesis of organic acid involvement in the solubilization of insoluble P by those isolates (Trivedi and Sa, 2008; Elizabeth et al., 2007; Chen et al., 2006; Hwangbo et al., 2003; Vazquez et al., 2000).”
Tricalcium phosphate is inappropriate as a universal selection factor for isolating and testing phosphate-solubilizing bacteria that enhance plant growth: a proposal for an alternative procedure (2013, background): “Pérez et al. (2007) showed that the latter is hardly solubilized (Kumar and Narula 1999).”
Solubilisation of inorganic phosphates by inoculant strains from tropical legumes (2011, background): “It is possible that a smaller amount of solubilisation may have occurred on the solid medium without the formation of the characteristic halo around the colony as observed by Perez et al. (2007).”