Quantitative Sequencing of 5-Methylcytosine and 5-Hydroxymethylcytosine at Single-Base Resolution

By M. Booth, Miguel R. Branco, G. Ficz, David Oxley, Felix Krueger, Wolf Reik, Shankar Balasubramanian. Science, 2012

839 citations recorded by the source corpus.

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

Reversing DNA Methylation: Mechanisms, Genomics, and Biological Functions (2014, positive): “Therefore, subtracting signals of oxBS-seq maps from standard BS-seq signals would reveal the location and abundance of 5hmC (Booth et al., 2012).”
Wilms tumor protein recognizes 5-carboxylcytosine within a specific DNA sequence (2014, neutral): “, 2009), 5hmC (Booth et al., 2012; Sun et al., 2013; Yu et al., 2012), and more recently 5fC (Booth et al.”
The Dynamics of Genome-wide DNA Methylation Reprogramming in Mouse Primordial Germ Cells (2012, background): “Also, it is unclear at this point if methylation present in the PGC founder population is first converted into 5-hydroxymethylcytosine (5hmC) and then lost by subsequent passive demethylation, as BS-Seq data sets do not distinguish between 5mC and 5hmC, and current techniques that allow for this dis”
Analysing and interpreting DNA methylation data (2012, background): “Disadvantages include the high cost of whole-genome resequencing and the difficulty of discriminating between 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) 144,145 .”