Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications

By R. Freshney. 2010

3,343 citations recorded by the source corpus.

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. (2015, background): “Nutrient consumption Nutrients in cell culture medium generally in great excess, medium exchange typically needed every 48 h to once a week (Freshney, 2010).”
Characterization of two grouper Epinephelus akaara cell lines: Application to studies of Singapore grouper iridovirus (SGIV) propagation and virus-host interaction (2009, methodology): “Slides were prepared by following the conventional drop–splash technique (Freshney, 1994).”
Adult pancreatic β-cells are formed by self-duplication rather than stem-cell differentiation (2004, methodology): “Pancreases were dissociated to a single-cell suspension essentially as describe”
Antibodies against the organic matrix in scleractinians: a new tool to study coral biomineralization (2005, background): “Comparison with histological sections has its limits, because the morphology and coloration of in vitro cultured cells may be markedly different from those growing in vivo (Phillips 1961; Freshney 1987; Grobstein 1965).”
Endometrial regenerative cells: A novel stem cell population (2007, methodology): “Karyotypic analysis was performed according to routine methods, briefly, ERC were treated with colchicine and stained with DAPI for the chromosome analysis [15].”