MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments

By Stephen A. Bustin, J. Beaulieu, J. Huggett, R. Jaggi, F. Kibenge, P. Olsvik, L. C. Penning, S. Toegel. BMC Molecular Biology, 2010

688 citations recorded by the source corpus.

The conclusions of thousands of peer-reviewed publications rely on data obtained using fluorescence-based quantitative real-time PCR technology. However, the inadequate reporting of experimental detail, combined with the frequent use of flawed protocols is leading to the publication of papers that may not be technically appropriate. We take the view that this problem requires the delineation of a more transparent and comprehensive reporting policy from scientific journals. This editorial aims to provide practical guidance for the incorporation of absolute minimum standards encompassing the key assay parameters for accurate design, documentation and reporting of qPCR experiments (MIQE précis) and guidance on the publication of pure 'reference gene' articles.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/62f7629230066e69f5c776da59f2c5a781a00107

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In Molecular Biology

1. Bartłomiej Kozera
2. E. Genersch
3. L. Gauthier
4. G. Budge
5. Marcin Rapacz
6. Matthias Mann

Citations in the corpus

• The need for robust qPCR‐based eDNA detection assays in environmental monitoring and species inventories (2020, background): “Robust eDNA assays include abiding by fundamental principles of qPCR best practices (e.g., MIQE; Bustin et al., 2010), while also requiring special considerations in their relationship within the eDNA survey workflow and application to often highly dilute and complex environmental samples.”
• Standard methods for molecular research in Apis mellifera (2013, methodology): “…PCR products reach a fluorescence detection threshold, read after each cycle by laser optics, can be very accurately related to the initial amount of target in the reaction, through the use of exponential algorithms and internal and external quantitation standards (Bustin et al., 2009; 2010).”
• Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “Although no perfect assessment method is postulated, microfluidics-based systems or 3’:5’ ratio assays are defined as basic indicators of RNA integrity (Bustin et al., 2010).”
• Reference genes in real-time PCR (2013, methodology): “…in Germany (March 2004), continued further on in different countries as lead by Prof. Stephen Bustin, and the publication of MIQE Guidelines which goes for Minimum Information for Publication of Quantitative Real-Time PCR Experiments (Bustin et al. 2009; Bustin et al. 2010; Derveaux et al. 2010).”