Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems

By K. Terpe. Applied Microbiology and Biotechnology, 2002

1,443 citations recorded by the source corpus.

Subfields: Molecular Biology, Oncology, Radiology, Nuclear Medicine and Imaging

View Paper: https://www.semanticscholar.org/paper/62cf465bbf50dae7906de5492d721452086c9744

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. S. Muyldermans
2. H. P. Sørensen
3. K. Mortensen
4. H. Leonhardt
5. Lukasz Kurgan
6. Fanchi Meng

In Oncology

1. H. P. Sørensen
2. K. Mortensen

In Radiology, Nuclear Medicine and Imaging

1. S. Muyldermans
2. H. P. Sørensen
3. K. Mortensen
4. Lukasz Kurgan
5. Fanchi Meng

Citations in the corpus

• Fusion Protein Linkers: Property, Design and Functionality (2012, background): “Besides their wide applications in biological research such as protein purification [1] and imaging [2], recombinant fusion proteins have also become an important category of biopharmaceuticals (Figure 1) [3, 4].”
• Overview of Affinity Tags for Protein Purification (2004, background): “Unfortunately, the phenylarsine oxide matrix used to purify thioredoxin fusion proteins often does not provide high-yield, high-purity product, likely because of nonspecific background protein binding (Terpe, 2003).”
• Recombinant protein expression and purification: A comprehensive review of affinity tags and microbial applications (2012, background): “[4] Terpe, K.”
• Advanced genetic strategies for recombinant protein expression in Escherichia coli. (2005, background): “Several other affinity tags exist and have been extensively reviewed (Terpe, 2003).”
• A Versatile Nanotrap for Biochemical and Functional Studies with Fluorescent Fusion Proteins*S (2008, background): “Those limitations are often bypassed by fusing the protein of interest to specific epitope or protein tags including hemagglutinin, c-Myc, FLAG, or GST (7, 8 ). Curiously GFP, the most widely used labeling tag in cell biology, is rarely used for biochemical analyses, although various mono- and poly”
• Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins. (2006, neutral): “Softag1, Softag 3 13, 8 Recognized by polyol-responsive mAb [46–48] c-myc 10 mAb-based puriWcation [31] T7-tag 11–16 mAb-based puriWcation [56]”