Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins.

By J. Arnau, C. Lauritzen, G. Petersen, J. Pedersen. Protein Expression and Purification, 2006

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Recombinant protein expression and purification: A comprehensive review of affinity tags and microbial applications (2012, background): “While contemporary reports have included several overviews of available affinity tags for protein detection or purification [1–4], solubility enhancement [5], tag removal [1], or applications [6, 7] – in this comprehensive review, we provide an extensive summary of various tags and established purif”
Application of enzymes for textile fibres processing (2008, background): “E-mail: artur@det.uminho.pt ISSN 1024-2422 print/ISSN 1029-2446 online # 2008 Informa UK Ltd DOI: 10.1080/10242420802390457 unrelated genera Bacillus, (Silbersack et al. 2006, Biedendieck et al. 2007) Clostridium (Girbal et al. 2005) Staphylococcus and the lactic acid bacteria Streptococcus (Arnau e”
Application of Ni(II)-Assisted Peptide Bond Hydrolysis to Non-Enzymatic Affinity Tag Removal (2012, background): “However, many applications require the removal of affinity tags after the purification step, since the tags often change conformation of proteins, alter their biological activity, or make them toxic [1].”
An Overview of Enzymatic Reagents for the Removal of Affinity Tags (2011, background): “coli has inspired the development of a generic protocol for protein purification [2,3] that is outlined in Fig.”