AREB1 Is a Transcription Activator of Novel ABRE-Dependent ABA Signaling That Enhances Drought Stress Tolerance in Arabidopsis[W][OA]

By Y. Fujita, M. Fujita, Rie Satoh, K. Maruyama, M. M. Parvez, Motoaki Seki, K. Hiratsu, M. Ohme-Takagi, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki. The Plant Cell Online, 2005

950 citations recorded by the source corpus.

ABSCISIC ACID–RESPONSIVE ELEMENT BINDING PROTEIN1 (AREB1) (i.e., ABF2) is a basic domain/leucine zipper transcription factor that binds to the abscisic acid (ABA)–responsive element (ABRE) motif in the promoter region of ABA-inducible genes. Here, we show that expression of the intact AREB1 gene on its own is insufficient to lead to expression of downstream genes under normal growth conditions. To overcome the masked transactivation activity of AREB1, we created an activated form of AREB1 (AREB1ΔQT). AREB1ΔQT-overexpressing plants showed ABA hypersensitivity and enhanced drought tolerance, and eight genes with two or more ABRE motifs in the promoter regions in two groups were greatly upregulated: late embryogenesis abundant class genes and ABA- and drought stress–inducible regulatory genes. By contrast, an areb1 null mutant and a dominant loss-of-function mutant of AREB1 (AREB1:RD) with a repression domain exhibited ABA insensitivity. Furthermore, AREB1:RD plants displayed reduced survival under dehydration, and three of the eight greatly upregulated genes were downregulated, including genes for linker histone H1 and AAA ATPase, which govern gene expression and multiple cellular activities through protein folding, respectively. Thus, these data suggest that AREB1 regulates novel ABRE-dependent ABA signaling that enhances drought tolerance in vegetative tissues.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/61ffb8eedc701a3afe53286acb4ac3f2d2652d65

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

In Plant Science

Citations in the corpus

Plant hormone-mediated regulation of stress responses (2016, background): “The basic leucine zipper transcription factors, ABRE-BINDING PROTEINS (AREBs)/ABRE-BINDING FACTORS (ABFs) can bind to ABRE and result in the upregulation of ABA-responsive genes [16].”
Abiotic and biotic stress combinations. (2014, background): “GBF3was shown to be involved inABA signaling and is responsive tomultiple stress conditions (Fujita et al., 2005).”
Molecular Basis of the Core Regulatory Network in ABA Responses: Sensing, Signaling and Transport (2010, background): “In addition, nine AREB/ABFs may be involved in ABA-responsive gene expression ( Uno et al. 2000 , Fujita et al. 2005 ), resulting in > 3,000 possible combinations for transcriptional regulation.”
Genetic engineering and breeding of drought-resistant crops. (2014, background): “Overexpression of AREB1/ABF2, ABF3, or AREB2/ABF4 in Arabidopsis enhanced ABA sensitivity and DT (27, 49).”
Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. (2006, background): “By contrast, an areb1 null mutant and a dominant loss-of-function mutant of AREB1 with a repression domain exhibited ABA insensitivity and some of the upregulated genes were downregulated (24).”
Regulatory metabolic networks in drought stress responses. (2007, background): “Current Opinion in Plant Biology 2007, 10:296–302 RESPONSIVE ELEMENT (ABRE)-BINDING FACTOR 2 (ABF2) [4,5] and DEHYDRATION-RESPONSIVE ELEMENT (DRE)-BINDING PROTEIN 2A (DREB2A) [6], and protein kinases, such as RECEPTOR-LIKE KINASE 1 (RPK1) [7], SNF1-RELATED PROTEIN KINASE 2C (SRK2C) [8] and the guard”
Inducing drought tolerance in plants: recent advances. (2010, background): “…al. (2004) Abscisic acid-responsive element-binding protein1 (AREB1) Arabidopsis thaliana L. Escherichia coli AREB1 regulated novel ABRE-dependent Fujita et al. (2005) ABA signaling that enhanced drought tolerance in vegetative tissues Zea mays ABA/ water stress/ripening induced protein…”
ABA signaling in stress-response and seed development (2013, background): “Among the group A bZIP subfamily members, AREB1/ABF2, AREB2/ABF4 and ABF3 are induced by dehydration, high salinity and ABA treatment in vegetative tissues (Fujita et al. 2005), and plants overexpressing these factors show enhanced drought tolerance (Kang et al.”
Genomic Survey and Gene Expression Analysis of the Basic Leucine Zipper Transcription Factor Family in Rice1[W][OA] (2007, background): “It is worth mentioning that a recent study reports that three bZIP transcription factor genes, AREB1/ABF2, AREB2/ABF4, and ABF3/DPBF5, depict ABA-, drought-, and high-salinity-inducible expression in vegetative tissues in Arabidopsis (Fujita et al., 2005).”
Three SnRK2 protein kinases are the main positive regulators of abscisic acid signaling in response to water stress in Arabidopsis. (2009, methodology): “Analysis of transient expression in onion epidermal cells was conducted as described previously ( Fujita et al. 2005 ).”
Signal transduction during cold, salt, and drought stresses in plants (2011, background): “Thus, these data suggest that AREB1 regulates novel ABRE-dependent ABA signaling that enhances drought tolerance in vegetative tissues [92].”
Transcription factors in plants and ABA dependent and independent abiotic stress signalling (2010, background): “ABF2/ AREB1 is an essential component of glucose signalling, and its overexpression increased tolerance to multiple stresses (Kim et al. 2004, Fujita et al. 2005).”
Improvement of drought resistance in rice. (2009, positive): “responsive genes without exogenous ABA application (Fujita et al., 2005; Furihata et al., 2006).”
Effects of abiotic stress on plants: a systems biology perspective (2011, background): “In the vegetative stage, expression of ABA-responsive genes is mainly regulated by bZIP transcription factors (TFs) known as AREB/ABFs, which act in an ABA-responsiveelement (ABRE) dependent manner [45-47].”
Guard Cell Signal Transduction Network: Advances in Understanding Abscisic Acid, CO2, and Ca2+ Signaling (2010, background): “Posttranslational modification of AREB1 might trigger induction of downstream ABA-responsive genes because enhanced general ABA responses were reported by overexpression of a constitutively active truncated form of AREB1 (36).”