Identification of a Novel Polyomavirus from Patients with Acute Respiratory Tract Infections
By A. Gaynor, M. Nissen, D. Whiley, I. Mackay, S. Lambert, Guang Wu, D. Brennan, G. Storch, T. Sloots, David Wang. PLoS Pathogens, 2007
715 citations recorded by the source corpus.
We report the identification of a novel polyomavirus present in respiratory secretions from human patients with symptoms of acute respiratory tract infection. The virus was initially detected in a nasopharyngeal aspirate from a 3-year-old child from Australia diagnosed with pneumonia. A random library was generated from nucleic acids extracted from the nasopharyngeal aspirate and analyzed by high throughput DNA sequencing. Multiple DNA fragments were cloned that possessed limited homology to known polyomaviruses. We subsequently sequenced the entire virus genome of 5,229 bp, henceforth referred to as WU virus, and found it to have genomic features characteristic of the family Polyomaviridae. The genome was predicted to encode small T antigen, large T antigen, and three capsid proteins: VP1, VP2, and VP3. Phylogenetic analysis clearly revealed that the WU virus was divergent from all known polyomaviruses. Screening of 2,135 patients with acute respiratory tract infections in Brisbane, Queensland, Australia, and St. Louis, Missouri, United States, using WU virus–specific PCR primers resulted in the detection of 43 additional specimens that contained WU virus. The presence of multiple instances of the virus in two continents suggests that this virus is geographically widespread in the human population and raises the possibility that the WU virus may be a human pathogen.
Subfields: Aerospace Engineering, Electrical and Electronic Engineering, Oncology
View Paper: https://www.semanticscholar.org/paper/61252de584faab4f92aed04624bdea593b842e33
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Aerospace Engineering
In Electrical and Electronic Engineering
In Oncology
Citations in the corpus
- NCBI Viral Genomes Resource (2014, background): “Whereas primary databases are archival repositories of sequence data, reference databases provide curated datasets that enable a number of activities, among them are transfer annotation to related genomes (11) (12) (13) , sequence assembly and virus discovery (14) (15) (16) (17) , viral dynamics and”
- Seroepidemiology of Human Polyomaviruses (2009, background): “Five human polyomaviruses have now been identified: BKV [1], JCV [2], KIV [3], WUV [4], and most recently, Merkel Cell Polyomavirus (MCV) [5].”
- Why do viruses cause cancer? Highlights of the first century of human tumour virology (2010, background): “The recent discovery of additional new human polyomaviruse”