Single-Molecule DNA Sequencing of a Viral Genome

By Timothy D. Harris, P. Buzby, H. Babcock, E. Beer, Jayson L Bowers, I. Braslavsky, Marie Causey, Jennifer Colonell, J. Dimeo, J. Efcavitch and 13 more. Science, 2008

709 citations recorded by the source corpus.

Subfields: Ecology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/602011691c7ebd347f14c264773c7491ac6a9227

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Citations in the corpus

De novo assembly of human genomes with massively parallel short read sequencing. (2010, methodology): “The development and commercialization of next-generation massively parallel DNA sequencing technologies, including Illumina Genome Analyzer (GA) (Bentley 2006), Applied Biosystems SOLiD System, and Helicos BioSciences HeliScope (Harris et al. 2008), have revolutionized genomic research.”
Noninvasive diagnosis of fetal aneuploidy by shotgun sequencing DNA from maternal blood (2008, background): “Both this problem and the sample-volume limitations may possibly be resolved by the use of single-molecule sequencing technologies, which do not require the use of PCR for library preparation (40).”
Applications of next generation sequencing in molecular ecology of non-model organisms (2011, methodology): “At the time of writing only the Helicos tSMS system (Harris et al., 2008) is commercially available but others (like the SMRT technology of Pacific Biosciences) are due to be launched later this year.”
Utilization of next-generation sequencing platforms in plant genomics and genetic variant discovery (2010, methodology): “Multiple cycles of single-base extension generate reads whose average length is 25 bps or greater (Harris et al. 2008).”
SNP Discovery through Next-Generation Sequencing and Its Applications (2012, background): “The ﬂuorescent tag on the incorporated nucleotide is then chemically cleaved to allow subsequent elongation of DNA [30].”
Sequencing technologies and genome sequencing (2011, methodology): “…the introduction of the first automated DNA sequencers led by Caltech (Smith et al. 1986), which was subsequently commercialized by Applied Biosystems (ABI), the European Molecular Biology Laboratory (EMBL) (Ansorge et al. 1986, 1987) and PharmaciaAmersham, later General Electric (GE) healthcare.”
Next‐generation sequencing technologies for environmental DNA research (2012, background): “Another cycle of single-base extension, label-cleaving and imaging follows (Ewing et al. 1998; Harris et al. 2008; Zhang et al. 2011).”
Noninvasive prenatal diagnosis of fetal chromosomal aneuploidy by massively parallel genomic sequencing of DNA in maternal plasma (2008, neutral): “The same approach can also be used for the other platforms (16, 27).”