Cell signalling by microRNA165/6 directs gene dose-dependent root cell fate

By Annelie Carlsbecker, Ji-Young Lee, Christina J Roberts, J. Dettmer, Satu J. Lehesranta, Jing Zhou, O. Lindgren, M. Moreno-Risueno, Anne Vatén, S. Thitamadee and 5 more. Nature, 2010

816 citations recorded by the source corpus.

A key question in developmental biology is how cells exchange positional information for proper patterning during organ development. In plant roots the radial tissue organization is highly conserved with a central vascular cylinder in which two water conducting cell types, protoxylem and metaxylem, are patterned centripetally. We show that this patterning occurs through crosstalk between the vascular cylinder and the surrounding endodermis mediated by cell-to-cell movement of a transcription factor in one direction and microRNAs in the other. SHORT ROOT, produced in the vascular cylinder, moves into the endodermis to activate SCARECROW. Together these transcription factors activate MIR165a and MIR166b. Endodermally produced microRNA165/6 then acts to degrade its target mRNAs encoding class III homeodomain-leucine zipper transcription factors in the endodermis and stele periphery. The resulting differential distribution of target mRNA in the vascular cylinder determines xylem cell types in a dosage-dependent manner.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/5e420a4a807353a841d3e9ce042a66cfcdc66b63

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In Plant Science

1. S. Lev-Yadun
2. Philip N. Benfey
3. J. Sperry
4. M. Grusak
5. Julie Kang
6. Uwe G Hacke
7. Tim J. Brodribb

Citations in the corpus

• Control of Arabidopsis root development. (2012, background): “(27) showed that after SHR moves from the stele into the endodermis, its activation of SCR allows SHR/SCR activation of microRNA165/166 (miR165/166) expression.”
• The widespread regulation of microRNA biogenesis, function and decay (2010, background): “However, there are examples of non-cell-autonomous miRNA actio”
• Plant Fiber Formation: State of the Art, Recent and Expected Progress, and Open Questions (2012, background): “The xylem fate promoting HB-ZIP III TFs are negatively regulated by miRNA165/166, which has been shown to regulate xylem differentiation in a dose-dependent manner in Arabidopsis roots (Carlsbecker et al., 2010).”
• The plant vascular system: evolution, development and functions. (2013, background): “A bidirectional cell-to-cell communication network involving the mobile transcription factor SHORTROOT (SHR) and microRNA165/166 species specifies the radial position of two types of xylem vessels in Arabidopsis roots (Carlsbecker et al. 2010; Miyashima et al. 2011).”
• Biological functions of microRNAs: a review (2011, background): “Endodermally produced miRNAs then act to degrade target mRNAs encoding class III homeodomain-leucine zipper transcription factors in the endodermis and stele periphery [ 5 ].”