Charting the proteomes of organisms with unsequenced genomes by MALDI-quadrupole time-of-flight mass spectrometry and BLAST homology searching.

By Andrej Shevchenko, Shamil R. Sunyaev, A. Loboda, A. Shevchenko, Peer Bork, W. Ens, K. Standing. Analytical Chemistry, 2001

583 citations recorded by the source corpus.

Subfields: Molecular Biology, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/5dd9d9b94bdbe75e44eb2b372bf0c10cfef20ea4

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Citations in the corpus

Trypsin Cleaves Exclusively C-terminal to Arginine and Lysine Residues*S (2004, methodology): “For other algorithms, this question has been addressed by analyzing defined mixtures of known proteins (3); or by searching in reversed databases that should not yield significant hits (4, 5).”
The Paragon Algorithm, a Next Generation Search Engine That Uses Sequence Temperature Values and Feature Probabilities to Identify Peptides from Tandem Mass Spectra*S (2007, background): “…category approaches that use automatic de novo sequencing and attempt to call larger stretches of sequence particularly as a solution to the so-called “homology searching” problem where it is expected that the proteins from the species of interest are poorly represented in the database (27–29).”
Abiotic Stress Responses and Microbe-Mediated Mitigation in Plants: The Omics Strategies (2017, background): “Recent advancements in protein sequencing are the key step for the identification of proteins from diverse species (Cordwell et al., 1995, 1997; Shevchenko et al., 2001).”
Characterisation of commercial papain, bromelain, actinidin and zingibain protease preparations and their activities toward meat proteins (2012, methodology): “The gel bands were then incubated for 30 min with 80% acetonitrile in 0.1% trifluoroacetic acid (Shevchenko et al., 2001).”
MATRIX-ASSISTED LASER DESORPTION MASS SPECTROMETRY (2002, background): “protein digestion,([29,30]) or acylation of the N-terminal fragmentation ions([31])”