Strategies for the assessment of matrix effect in quantitative bioanalytical methods based on HPLC-MS/MS.

By B. Matuszewski, M. Constanzer, Chávez Cm. Analytical Chemistry, 2003

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Matrix effects: the Achilles heel of quantitative high-performance liquid chromatography-electrospray-tandem mass spectrometry. (2005, methodology): “[21] have elegantly shown the influence of different subjects on assay imprecision for a quantitative HPLC–ESI–MS/MS method by comparing standard samples prepared from one pool of plasma with that from multiple sources.”
Metabolite profiling: from diagnostics to systems biology (2004, background): “Whereas gas-chromatography-based approaches can only be used with volatile compounds or compounds that can be rendered volatile by derivitization, LC–MS can be adapted to a wider array of molecules, including a range of secondary metabolites such as alkaloids, flavonoids, glucosinolates, isoprenes, ”
Validation of new methods. (2007, methodology): “However, no matter which approach is used in validation studies, it is essential to evaluate several sources of blank matrix [18,19] just as it has been described for the selectivity experiments.”