Automatic and quantitative measurement of protein-protein colocalization in live cells.

By S. Costes, D. Daelemans, Edward H. Cho, Zachary C. Dobbin, G. Pavlakis, S. Lockett. Biophysical Journal, 2004

1,478 citations recorded by the source corpus.

Subfields: Biophysics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/594fd2cdafd5b1554c0c458d039d5ab0113daa7d

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In Biophysics

1. S. Bolte
2. Fabrice Cordelières
3. M. Saxton
4. Erik H. W. Meijering

In Molecular Biology

1. T. von Zglinicki
2. J. Passos
3. Malcolm J. Bennett
4. R. Swarup
5. Yoseph Barash
6. I. Slaninová

Citations in the corpus

• ImageJ for microscopy. (2007, background): “Among others, plugins are available to perform qualitative overlays [and convert them from Red-Green-Blue (RGB) to the color-blind-friendly Magenta-Green- Blue], generate Pearson's coefficients, generate Mander's coefficients, and perform various randomization co-localization tests [e.g., Fay (1), C”
• A guided tour into subcellular colocalization analysis in light microscopy (2006, methodology): “(2003) tested the significance of the colocalization results against the degree of colocalization in randomized images, produced as already described (Costes et al., 2004).”
• Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK (2017, neutral): “Thresholds were set automatically by the software using the method of Costes et al (2004)30.”
• Quantifying colocalization by correlation: The Pearson correlation coefficient is superior to the Mander's overlap coefficient (2010, methodology): “Automatic thresholding, using the idea that the background pixels are uncorrelated (16) or based on the background mean and standard deviation, are alternatives to operator controlled thresholding.”
• Convergence of Acquired Mutations and Alternative Splicing of CD19 Enables Resistance to CART-19 Immunotherapy (2015, background): “Statistical Costes P value is 1 for this analysis (42).”
• Calpain-mediated cleavage of Atg5 switches autophagy to apoptosis (2006, methodology): “For quantitative analysis of colocalization, the Pearson's correlation coefficient was calculate”
• Colocalization of fluorescent markers in confocal microscope images of plant cells (2008, methodology): “Other methods additionally use probabilistic models to be able to make a certainty judgment about the existence of colocalizatio”
• Mitochondrial Dysfunction Accounts for the Stochastic Heterogeneity in Telomere-Dependent Senescence (2007, methodology): “For co-localisation analysis, confocal images were deconvolved and analysed using Costes approximation method [73] in ImageJ, v1.”