Beyond editing: repurposing CRISPR–Cas9 for precision genome regulation and interrogation

By Antonia Dominguez, Wendell A. Lim, Lei S. Qi. Nature reviews. Molecular cell biology, 2015

1,257 citations recorded by the source corpus.

The bacterial CRISPR–Cas9 system has emerged as a multifunctional platform for sequence-specific regulation of gene expression. This Review describes the development of technologies based on nuclease-deactivated Cas9, termed dCas9, for RNA-guided genomic transcription regulation, both by repression through CRISPR interference (CRISPRi) and by activation through CRISPR activation (CRISPRa). We highlight different uses in diverse organisms, including bacterial and eukaryotic cells, and summarize current applications of harnessing CRISPR–dCas9 for multiplexed, inducible gene regulation, genome-wide screens and cell fate engineering. We also provide a perspective on future developments of the technology and its applications in biomedical research and clinical studies.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/58729fa6ff91e20a59b34da09850849da50b2dfb

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Citations in the corpus

Triglyceride-rich lipoproteins and their remnants: metabolic insights, role in atherosclerotic cardiovascular disease, and emerging therapeutic strategies—a consensus statement from the European Atherosclerosis Society (2021, background): “…VLDL assembly and secretion are upregulated by (i) the delivery of FA released from insulin-resistant adipocytes, (ii) the increased delivery of remnant TG–FA to the liver due to reduced peripheral lipolysis of chylomicron and VLDL–TG, and (iii) the increased de novo lipogenesis 55 ( Figure 4 ).”
Multiplex gene editing by CRISPR-Cpf1 through autonomous processing of a single crRNA array (2016, background): “Multiplex genome editing provides a powerful tool for targeting members of multigene families, dissecting gene networks, and modeling multigenic disorders in vivo (Dominguez et al., 2016; Heidenreich and Zhang, 2015; Hsu et al., 2014).”
CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture. (2019, background): “, VP64, p65AD, and VPR) can be used for gene regulation (26) (Figure 2c).”
Liver fibrosis: Pathophysiology, pathogenetic targets and clinical issues. (2019, background): “These nuclear receptors, which regulate energetic fluxes and metabolic pathways, are dysregulated in CLD, particularly in progressive NAFLD (Machado et al., 2014; Wang et al., 2015), and have been reported to inhibit HSC activation and fibrosis progression.”
The Post-GWAS Era: From Association to Function. (2018, background): “Thus, genome editing technologies can also be used to validate potential CREs by altering epigenetic state, rather than the underlying DNA sequence.(66,67) A more recent study used CRISPR/Cas9 gene editing to investigate candidate causal variants at the SNCA locus,(68) which is associated with risk ”