Human Genome Sequencing Using Unchained Base Reads on Self-Assembling DNA Nanoarrays

By R. Drmanac, Andrew B. Sparks, M. Callow, Aaron L. Halpern, Norman L. Burns, B. Kermani, P. Carnevali, Igor Nazarenko, Geoffrey B. Nilsen, George Yeung and 55 more. Science, 2010

1,234 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/5619871678b51a27f7f16b935612452556590d25

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Citations in the corpus

Robust and scalable inference of population history from hundreds of unphased whole-genomes (2016, methodology): “The Complete Genomics data are derived from 54 unrelated individuals obtained from various populations and have average coverage in excess of 45×.”
Metagenomics - a guide from sampling to data analysis (2012, background): “Complete Genomics is offering a technology based on sequencing DNA nanoballs with combinatorial probe-anchor ligation [36].”
Annotation of functional variation in personal genomes using RegulomeDB (2012, background): “We examined the cell line GM12878, a European female, which has been deeply sequenced as part of the 1000 Genomes Project and is included in the Complete Genomics samples.”
The sequence of sequencers: The history of sequencing DNA (2016, background): “…is novel: instead of a bead or bridge ampli ﬁ cation, rolling circle ampli ﬁ cation is used to generate long DNA chains consisting of repeating units of the template sequence bordered by adapters, which then self assemble into nanoballs, which are af ﬁ xed to a slide to be sequenced [68].”
Approximation to the Distribution of Fitness Effects across Functional Categories in Human Segregating Polymorphisms (2014, neutral): “To map C-scores to selective coefficients, we obtained allele frequency information from 9 Yoruba (YRI) 104 individuals (18 haploid sequences) sequenced to high-coverage using whole-genome shotgun sequencing 105 as part of a dataset produced by Complete Genomics (CG) [30].”
An integrated map of genetic variation from 1,092 human genomes (2012, neutral): “Samples 1,092 1,092 1,092 Total raw bases (Gb) 19,049 804 327 Mean mapped depth (3) 5.”
High‐throughput DNA sequencing – concepts and limitations (2010, methodology): “Their technology is also based on the Church lab sequencing-byligation concept, but combines it with a new strategy of sequencing library construction and sequence immobilization using rolling circle amplification [45].”
Characterizing and measuring bias in sequence data (2013, methodology): “Complete Genomics [29] also uses amplification along with a complex library construction process.”
Sequencing technologies — the next generation (2010, result): “The number of SNVs identified was consistent with those reported in Table 1 for other genomes, and the group reported an amortized cost of USd4,400 in reagent”
Nanopore sensors for nucleic acid analysis. (2011, methodology): “, sequencing by ligation on self-assembled DNA nanoarrays (Complete Genomics”
Integrative Annotation of Variants from 1092 Humans: Application to Cancer Genomics (2013, methodology): “For the 7,815 phase1 SNPs in ultrasensitive regions, we validated their existence using publicly available data from Complete Genomics (CG) (61).”