Conjugated linoleic acid supplementation reduces adipose tissue by apoptosis and develops lipodystrophy in mice.

By N. Tsuboyama-Kasaoka, Mayumi Takahashi, K. Tanemura, Hyoun-ju Kim, T. Tange, H. Okuyama, M. Kasai, S. Ikemoto, O. Ezaki. Diabetes, 2000

602 citations recorded by the source corpus.

Conjugated linoleic acid (CLA) is a naturally occurring group of dienoic derivatives of linoleic acid found in beef and dairy products. CLA has been reported to reduce body fat. To examine the mechanism(s) of CLA reduction of fat mass, female C57BL/6J mice were fed standard semipurified diets (10% fat of total energy) with or without CLA (1% wt/wt). Terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick endlabeling (TUNEL) and DNA fragmentation analysis revealed that fat-mass decrease by CLA was mainly due to apoptosis. Tumor necrosis factor (TNF)-alpha and uncoupling protein (UCP)-2 mRNA levels increased 12- and 6-fold, respectively, in isolated adipocytes from CLA-fed mice compared with control mice. Because it is known that TNF-alpha induces apoptosis of adipocytes and upregulates UCP2 mRNA, a marked increase of TNF-alpha mRNA with an increase of UCP2 in adipocytes caused CLA-induced apoptosis. However, with a decrease of fat mass, CLA supplementation resulted in a state resembling lipoatrophic diabetes: ablation of brown adipose tissue, a marked reduction of white adipose tissue, marked hepatomegaly, and marked insulin resistance. CLA supplementation decreased blood leptin levels, but continuous leptin infusion reversed hyperinsulinemia, indicating that leptin depletion contributes to the development of insulin resistance. These results demonstrate that intake of CLA reduces adipose tissue by apoptosis and results in lipodystrophy, but hyperinsulinemia by CLA can be normalized by leptin administration.

Subfields: Epidemiology, Nutrition and Dietetics, Physiology

View Paper: https://www.semanticscholar.org/paper/54d3a92fbcbcd1287498c3cdbae99290ab6e8704

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Citations in the corpus

A review of fatty acid profiles and antioxidant content in grass-fed and grain-fed beef (2010, background): “These changes in body composition occur at ultra high doses of CLA, dosages that can only be attained through synthetic supplementation that may also produce ill side-effects, such as gastrointestinal upset, adverse changes to glucose/insulin metabolism and compromised liver function [77-81].”
Biological effects of conjugated linoleic acids in health and disease. (2006, background): “In contrast, intake of 1% CLA in C57BL6 female mice fed with semipurified diet containing 4 g fat/100 g diet significantly decreased body fat mass with symptoms of lipoatrophic diabetes like IR and hepatomegaly [32].”