Continuous and Discontinuous RNA Synthesis in Coronaviruses
By I. Sola, F. Almazán, S. Zúñiga, L. Enjuanes. Annual Review of Virology, 2015
598 citations recorded by the source corpus.
Replication of the coronavirus genome requires continuous RNA synthesis, whereas transcription is a discontinuous process unique among RNA viruses. Transcription includes a template switch during the synthesis of subgenomic negative-strand RNAs to add a copy of the leader sequence. Coronavirus transcription is regulated by multiple factors, including the extent of base-pairing between transcription-regulating sequences of positive and negative polarity, viral and cell protein–RNA binding, and high-order RNA-RNA interactions. Coronavirus RNA synthesis is performed by a replication-transcription complex that includes viral and cell proteins that recognize cis-acting RNA elements mainly located in the highly structured 5′ and 3′ untranslated regions. In addition to many viral nonstructural proteins, the presence of cell nuclear proteins and the viral nucleocapsid protein increases virus amplification efficacy. Coronavirus RNA synthesis is connected with the formation of double-membrane vesicles and convoluted membranes. Coronaviruses encode proofreading machinery, unique in the RNA virus world, to ensure the maintenance of their large genome size.
Subfields: Animal Science and Zoology, Infectious Diseases
View Paper: https://www.semanticscholar.org/paper/54088756adb6dca571dfc4ea1d6d7384a15f4e5e
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Infectious Diseases
Citations in the corpus
- Current Status of Epidemiology, Diagnosis, Therapeutics, and Vaccines for Novel Coronavirus Disease 2019 (COVID-19) (2020, background): “During replication, coronaviruses generate 6-9 subgenomic mRNAs (sgmRNAs), which lead to translation of accessory and structural proteins from downstream ORFs [3].”
- Genotype and phenotype of COVID-19: Their roles in pathogenesis (2020, background): “Coronavirus genome replication and tran scription takes place at cytoplasmic membranes and involve coordinated processes of both continuous and discontinuous RNA synthesis that are mediated by the viral replicate, huge protein complex encoded by the 20-kb replicase gene(12).”
- The Architecture of SARS-CoV-2 Transcriptome (2020, unknown): “Each coronaviral RNA contains the common 50 ‘‘leader’’ sequence of 70 nt fused to the ‘‘body’’ sequence from the downstream part of the genome (Lai and Stohlman, 1981; Sola et al., 2015) (Figure 1).”