New primers to amplify the fungal ITS2 region--evaluation by 454-sequencing of artificial and natural communities.
By K. Ihrmark, Inga T M Bödeker, Karelyn Cruz-Martínez, H. Friberg, Ariana Kubartová, J. Schenck, Ylva Strid, J. Stenlid, Mikael Brandström-Durling, K. Clemmensen and 1 more. FEMS Microbiology Ecology, 2012
1,875 citations recorded by the source corpus.
Subfields: Cell Biology, Molecular Biology, Plant Science
View Paper: https://www.semanticscholar.org/paper/5344079882c0b7a25ac0d35486d486b539a43db3
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In Cell Biology
- Leho Tedersoo
- M. Bahram
- Kessy Abarenkov
- Urmas Kõljalg
- Peer Bork
- Ian A. DICKIE
- Petr Baldrian
- Serita Frey
In Molecular Biology
- Leho Tedersoo
- S. Morales
- M. V. D. van der Heijden
- Kessy Abarenkov
- Urmas Kõljalg
- K. Stinson
- Peer Bork
- Ian A. DICKIE
In Plant Science
- Leho Tedersoo
- M. Bahram
- Kessy Abarenkov
- Urmas Kõljalg
- A. Jumpponen
- M. V. D. van der Heijden
- K. Stinson
- Peer Bork
Citations in the corpus
- Cropping practices manipulate abundance patterns of root and soil microbiome members paving the way to smart farming (2018, methodology): “The ITS amplicon library was generated using the PCR primers fITS7 [74] and ITS4 [75].”
- Forest microbiome: diversity, complexity and dynamics. (2016, background): “Their absence in surveys was likely due to biases in themost frequently usedmolecularmarkers (Ihrmark et al. 2012; Baldrian et al. 2013b; Větrovský et al. 2016).”
- Soil bacterial networks are less stable under drought than fungal networks (2018, neutral): “For bacteria, the V3 – V4 hypervariable regions of the 16S rRNA gene were targeted using primers 341F 51 and 806R 52 ; for fungi, the ITS2 region was ampli fi ed using fITS7f and ITS4r primer sequences described by Ihrmark et al. 53 .”
- Biological, physicochemical and plant health responses in lettuce and strawberry in soil or peat amended with biochar (2016, positive): “To amplify the fungal rDNA-ITS2 region an adapted forward primer of fITS7bis from Ihrmark et al. (2012) (GTGAATCATCRAATYTTTG) and the ITS4NGSr reverse primer (Tedersoo et al., 2014) were used, both extended with Illumina speci fi c adaptors.”
- Rare microbial taxa as the major drivers of ecosystem multifunctionality in long-term fertilized soils (2020, neutral): “, 2015) and gITS7/ITS4 (Ihrmark et al., 2012), respectively.”